Methionine Oxidation and Proline cis-trans Isomerization in α_<s1>-Casein on Emulsification with Oxidized Lipid(Biological Chemistry)

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概要

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• An emulsion was prepared by homogenizing soybean oil with an α_<s1>-casein solution. The α_<s1>-casein adsorbed to the oil/water interface in the emulsion was digested with trypsin and then analyzed by reversed-phase high-performance liquid chromatography. Two peaks, T-1 and T-2E, due to the same fragment (^<194>Thr-Thr-Met-Pro-Leu-Trp) were found. Amino acid analysis showed that the ^<196>Met of T-1 had been oxidized to methionine sulfoxide, and the NMR spectrum indicated that the ^<196>Met-Pro bond of T-1 had been subjected to cis-trans isomerization. The isomerization of the same peptide bond was also observed when then peptide fragment was oxidized by chloramine-T in solution, suggesting that cis-trans isomerization of ^<197>Pro was induced by ^<196>Met oxidation.

• 社団法人日本農芸化学会の論文
• 1987-02-23

著者

• SHIMIZU Makoto

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• YAMAUCHI Kunio

  Department of Agricultural Chemistry, The University of Tokyo

• TAKAHASHI Seizo

  Department of Materials and Biological Sciences, Faculty of Science, Japan Women's University

• KAMINOGAWA Shuichi

  Department of Food Science and Technology, College of Bioresource Sciences, Nihon University

• Takahashi Seizo

  Department Of Chemistry Japan Women's University

• Takahashi Seizo

  Department Of Chemical And Biological Sciences Japan Women's University

• AMETANI Akio

  Applied Biological Chemistry, University of Tokyo

• AMETANI Akio

  Department of Applied Biological Chemistry, The University of Tokyo

• Kametani Y

  Applied Biological Chemistry University Of Tokyo

• Ametani Akio

  Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)torrey Pines Ins

• Yamauchi K

  Univ. Tokyo Tokyo Jpn

• Yamauchi Kunio

  Department Of Agricultural Chemistry The University Of Tokyo

• Kaminogawa S

  Dep. Of Food Sci. And Technol. Nihon Univ.

• Shimizu M

  Department Of Physics Faculty Of Science Tohoku University

• Kaminogawa Shuichi

  Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo

• Shimizu Makoto

  Department Of Applied Biological Chemistry The University Of Tokyo

• Shimizu Makoto

  Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo

• Takahashi Seizo

  Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo

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