Occurrence of High Molecular Weight EGF Complexes in Human Milk(Food & Nutrition)
スポンサーリンク
概要
- 論文の詳細を見る
A survey was conducted of the cell-growth-promoting factors in human milk using anti-human EGF antiserum and by a DNA synthesis assay in BALB/c mouse 3T3 embryo fibroblast cells. Three fractions having affinity toward anti-human EGF antibody were found by gel filtration on Sephacryl S-300. The fraction eluted at the void volume and the one with a molecular weight of about 70,000 both showed weak DNA synthesis stimulatory activity. On the other hand, the one with a molecular weight of about 6,000 highly stimulated DNA synthesis. This fraction was thought to contain EGF, whose molecular weight is about 6,000. Rechromatography by gel permeation again separated both the two high molecular weight peaks into 3 peaks and produced similar chromatograms. The results suggest that these 3 fractions may have been in an apparent equilibrium state. Considering that the EGF-fraction of molecular weight 6,000 did not show self-association, it is presumed that an EGF binding protein exists in human milk and forms higher molecular weight EGF complexes.
- 社団法人日本農芸化学会の論文
- 1989-04-23
著者
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Azuma Norihiro
Department of Bioproductive Science, Faculty of Agriculture, Utsunomiya University
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YAMAUCHI Kunio
Department of Agricultural Chemistry, The University of Tokyo
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KAMINOGAWA Shuichi
Department of Food Science and Technology, College of Bioresource Sciences, Nihon University
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Kaminogawa Shuichi
Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th
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Yamauchi K
Faculty Of Agriculture Miyazaki University
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Yamauchi Kunio
Department Of Agricultural Chemistry The University Of Tokyo
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Azuma N
Department Of Biological Resource Chemistry Kyoto Prefectural University
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Azuma Norihiro
Department Of Agricultural Chemistry The University Of Tokyo
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Kaminogawa S
Dep. Of Food Sci. And Technol. Nihon Univ.
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HESAKA Eri
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Yamauchi K
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Kaminogawa Shuichi
Department Of Agricultural Chemistry Faculty Of Engineering The University Of Tokyo
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Hesaka Eri
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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