Modified Keratin Sponge : Binding of Bone Morphogenetic Protein-2 and Osteoblast Differentiation(MEDICAL BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
A keratin sponge was chemically modified to obtain carboxyl and amino sponges by the alkylation of a large amount of active SH group on keratin proteins with iodoacetic acid and 2-bromoethylamine, respectively. The carboxyl sponge having a large amount of carboxyl group was a scaffold that could bind significant amounts of basic bioactive proteins, such as lysozyme and bone morphogenetic protein (BMP)-2, and drugs. Lysozyme (maximum 3.7mg), a model of basic cytokines such as BMP-2, was absorbed by the carboxyl sponge (4.8mg), but not by the amino sponge. The lysozyme was rapidly released from the carboxyl sponge in a buffer containing at a concentration higher than 0.5M, but at 0.15M, near the physiological ionic strength, after initial burst (only 11%), no significant release was observed (15%, 48h). BMP-2 also bound the carboxyl sponge. The preosteoblast cells grown inside the BMP-2-loaded sponge differentiated, whereas those grown outside the sponge did not, suggesting that no significant amount of BMP-2 leaked into the surrounding media. The effects of BMP-2 were confined inside modified keratin sponge. Therefore, using in vivo, we expect that only internal osteogenesis will be induced and that no external heterotopis ossification will be induced.
- 社団法人日本生物工学会の論文
- 2006-11-25
著者
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TACHIBANA Akira
Department of Biological Science, Faculty of Science, Ibaraki University
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NISHIKAWA Yuji
Department of Pathology, Akita University School of Medicine
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山内 清
大阪市大 工
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Yamauchi Kiyoshi
Department of Biology, Faculty of Science, Shizuoka University
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Tanabe Toshizumi
Department of Bioengineering, Graduate School of Engineering, Osaka City University
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Nishikawa Yuji
Department Of Bioengineering Graduate School Of Engineering Osaka City University
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Akira Tachibana
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Tachibana Akira
Department Of Biology Faculty Of Science Osaka City University
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Tachibana Akira
Department Of Bioengineering Graduate School Of Engineering Osaka City University
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Yamauchi K
Faculty Of Agriculture Miyazaki University
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Nishino Masaaki
Department of Bioengineering, Graduate School of Engineering, Osaka City University
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Kaneko Sumika
Department of Bioengineering, Graduate School of Engineering, Osaka City University
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Nishino Masaaki
Department Of Bioengineering Graduate School Of Engineering Osaka City University
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Tanabe T
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Tanabe Toshizumi
Department Of Bioengineering Graduate School Of Engineering Osaka City University
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Kaneko Sumika
Department Of Bioengineering Graduate School Of Engineering Osaka City University
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Yamauchi K
Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive
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Tachibana Akira
Rad. Biol. Canter Kyoto Univ.
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Yamauchi Kiyoshi
Department Of Bioapplied Chemistry Faculty Of Engineering Osaka City University
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Tachibana Akira
Rad. Biol. Center Kyoto Univ.
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TACHIBANA Akira
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City University
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Tanabe Toshizumi
Department of Applied Chemistry & Bioengineering, Graduate School of Engineering, Osaka City University
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