Identification of Thyroid Hormone-Responsive Genes in a Chicken Hepatoma Cell Line, LMH(Endocrinology)

概要

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Thyroid hormone receptors (TRs) are ligand-dependent transcription factors that regulate the transcription od\f multiple thyroid hormone (TH)-responsive genes. Our study aimed to indentifyTH-responsive genes in as estogen-responsive chicken hepatoma cell line, LMH, RNA was prepared from cells treated with or without 10^8 M 3,3,5-triodothyronine (T_3) for 24 h and was analyzed by differential displays. At least six cDNAs were detected whose transcript increased in the presence of T_3, and four of them were cloned. The four candidate TH-responsive genes that were indentified had high similarity (>83%) to known chichken or mammalian genes, which included the ribosomal protein L7 gene; the cytoplasmic nuclear ribonucleoprotein U (hnRNP U); and a gene for an unknown protein. Real-time PCR confirmed that the transcription of the four genes was responsive to T_3; their transcripts levels increased from four to eleven times with the administration of T3. The amount of TRβ transcript did not change with the administration of T_3. The physiological reasons for the activation of these genes and the utility od this cell line are discussed.
社団法人日本動物学会の論文
2005-10-25