Isolation and Characterization of a β-Primeverosidase Concerned with Alcoholic Aroma Formation in Tea Leaves

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概要

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• A β-primeverosidase concerned with alcoholic aroma formation during tea processing was purified from cv. Yabukita (Camellia sinensis var. sinensis). The acetone powder was subjected to ammonium sulfate precipitation and then to chromatographic purification with CM-Toyopearl 650M to give three active fractions, Glucosidases (Gls)-I, II, and III fractions. The main Gl-II fraction was characterized as a β-primeverosidase, which hydrolyzed a β-primeveroside into an aglycone and a primeverose (6-O-β-D-xylopyranosyl-β-D-glucopyranose) and was mainly concerned with alcoholic tea aroma formation. The Gl-II fraction was further seperated into Gls-IIa (main) and IIb (minor) by ion-exchange Mono S column chromatography. Gl-IIa was determined to be a monomeric protein of 61 kDa with pI 9.4. Gl-IIa was stable at temperature lower than 45℃ and at between pH 5 and 7, and showed its highest activity at 50℃ and at pH 5.

• 社団法人日本農芸化学会の論文
• 1996-11-23

著者

• WATANABE Naoharu

  Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

• 山内 清

  大阪市大 工

• Watanabe N

  Faculty Of Agriculture Shizuoka University

• Watanabe Naoharu

  Department Of Agricultural Chemistry The University Of Tokyo

• OGAWA Kenji

  Department of Surgery, Daini Hospital, Tokyo Women's Medical University

• Usui T

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• SAKATA Kanzo

  Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

• Usui Takeo

  Department Of Biotechnology Faculty Of Agriculture And Life Science University Of Tokyo

• USUI Taiichi

  Departmentof Environmental Science for Human Life, Faculty of Agriculture, Shizuoka University

• Luo Shaojun

  Hangzhou Tea Research Institute Ministry Of Internal Trade

• GUO Wenfei

  United Graduate School of Agricultural Sciences, Gifu University (Shizuoka University)

• YAMAUCHI Kazuyo

  Department of Applied Bio logical Chemistry, Faculty of Agriculture, Shizuoka University

• Sugiyama K

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• Sugiyama Kimio

  岐阜大学 連合農学研究科

• Guo Wenfei

  Faculty Of Agriculture Shizuoka University:(present Address)institute For Chemical Research Kyoto Un

• Usui T

  Dep. Of Applied Biological Chemistry Fac. Of Agriculture Shizuoka Univ.

• Usui Taiichi

  Departmentof Environmental Science For Human Life Faculty Of Agriculture Shizuoka University

• Sakata Kanzo

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• Sakata Kanzo

  Department Of Agricultural Chemistry Shizuoka University

• Yamauchi K

  Faculty Of Agriculture Miyazaki University

• Ogawa Kenji

  Department Of Orthopaedic Surgery Faculty Of Medicine Saga University

• Yamauchi K

  Department Of Applied Chemistry & Bioengineering Graduate School Of Engineering Osaka City Unive

• Sakata K

  Institute For Chemical Research Kyoto University

• Yamauchi Kiyoshi

  Department Of Bioapplied Chemistry Faculty Of Engineering Osaka City University

• Ogawa Kenji

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• Usui Taiichi

  Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University

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