Isolation and Characterization of a Novel Elastase Inhibitor, AFLEI from Aspergillus flavus
スポンサーリンク
概要
- 論文の詳細を見る
A novel elastase inhibitor from Aspergillus flavus (AFLEI) was isolated, and biochemical properties of AFLEI were examined. Column chromatography using diethylaminoethyl (DE) 52-Cellulose and Sephadex G-75 was used to purify the inhibitor. The final preparation was found to be homogeneous as indicated by a single band after disc polyacrylamide gel (PAGE) and isoelectric focusing electrophoreses. AFLEI had a molecular weight of 7,525.8 as determined by TOF-MS (time of flight mass spectrometry). The elastolytic activity of elastases from A. flavus, A. fumigatus and human leukocytes were inhibited by AFLEI. However, this activity from porcine pancreas elastase, trypsin, chymotrypsin, thrombin, and Ac1-Proteinase from snake venom was not affected by AFLEI. The fibrinogenase activity of the elastase from A. flavus was inhibited by AFLEI. AFLEI was inhibited by α2-macroglobulin. However, ethylenediaminetetraacetic acid (EDTA-2Na), benzamidine, chymostatin, tosyl phenylalanine chloromethyl ketone (TPCK) and dithiothreitol (DTT) did not show any inhibitory effect on the elastase inhibitory activity of AFLEI.
- 2006-07-31
著者
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OGAWA Kenji
Department of Surgery, Daini Hospital, Tokyo Women's Medical University
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Ogawa Kenji
Department Of Pulmonary Medicine Higashi Nagoya National Hospital
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OKUMURA Yoshiyuki
Department of Validation, Matsuurayakugyo Co., Ltd.
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UCHIYA Kei-ichi
Department of Microbiology, Faculty of Pharmacy, Meijo University
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NIKAI Toshiaki
Department of Microbiology, Faculty of Pharmacy, Meijo University
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Ogawa Kenji
Department Of Orthopaedic Surgery Faculty Of Medicine Saga University
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Nikai Toshiaki
Department Of Microbiology Faculty Of Pharmacy Meijo University
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Uchiya Kei-ichi
Department Of Microbiology Faculty Of Pharmacy Meijo University
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Okumura Yoshiyuki
Department Of Validation Matsuurayakugyo Co. Ltd
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Ogawa Kenji
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Okumura Yoshiyuki
Department of Quality Control, Mathuurayakugyo Co., Ltd.
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