Isolation and Characterization of a Novel Elastase Inhibitor, AFLEI from Aspergillus flavus
スポンサーリンク
概要
- 論文の詳細を見る
A novel elastase inhibitor from Aspergillus flavus (AFLEI) was isolated, and biochemical properties of AFLEI were examined. Column chromatography using diethylaminoethyl (DE) 52-Cellulose and Sephadex G-75 was used to purify the inhibitor. The final preparation was found to be homogeneous as indicated by a single band after disc polyacrylamide gel (PAGE) and isoelectric focusing electrophoreses. AFLEI had a molecular weight of 7,525.8 as determined by TOF-MS (time of flight mass spectrometry). The elastolytic activity of elastases from A. flavus, A. fumigatus and human leukocytes were inhibited by AFLEI. However, this activity from porcine pancreas elastase, trypsin, chymotrypsin, thrombin, and Ac1-Proteinase from snake venom was not affected by AFLEI. The fibrinogenase activity of the elastase from A. flavus was inhibited by AFLEI. AFLEI was inhibited by α2-macroglobulin. However, ethylenediaminetetraacetic acid (EDTA-2Na), benzamidine, chymostatin, tosyl phenylalanine chloromethyl ketone (TPCK) and dithiothreitol (DTT) did not show any inhibitory effect on the elastase inhibitory activity of AFLEI.
- 2006-07-31
著者
-
OGAWA Kenji
Department of Surgery, Daini Hospital, Tokyo Women's Medical University
-
Ogawa Kenji
Department Of Pulmonary Medicine Higashi Nagoya National Hospital
-
OKUMURA Yoshiyuki
Department of Validation, Matsuurayakugyo Co., Ltd.
-
UCHIYA Kei-ichi
Department of Microbiology, Faculty of Pharmacy, Meijo University
-
NIKAI Toshiaki
Department of Microbiology, Faculty of Pharmacy, Meijo University
-
Ogawa Kenji
Department Of Orthopaedic Surgery Faculty Of Medicine Saga University
-
Nikai Toshiaki
Department Of Microbiology Faculty Of Pharmacy Meijo University
-
Uchiya Kei-ichi
Department Of Microbiology Faculty Of Pharmacy Meijo University
-
Okumura Yoshiyuki
Department Of Validation Matsuurayakugyo Co. Ltd
-
Ogawa Kenji
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
-
Okumura Yoshiyuki
Department of Quality Control, Mathuurayakugyo Co., Ltd.
関連論文
- A Case of Diabetic Mastopathy with Multiple Lesions Mimicking Breast Cancer
- Adenocarcinoid of the Appendix : Report of Two Cases
- Gastric Metastasis of Breast Cancer in a Male Patient: Report of a Case
- A case of a cellular schwannoma variant arisen in an intrapelvic space
- Genetic Prognostic Index Influences Patient Outcome for Node-Positive Breast Cancer
- Upregulation and Overexpression of Human X-box Binding Protein 1 (hXBP-1) Gene in Primary Breast Cancers
- Copper-transporting P-Type Adenosine Triphosphatase (ATP7B) Is Expressed in Human Breast Carcinoma
- Unruptured pseudoaneurysm of the cystic artery with acute calculous cholecystitis incidentally detected by computed tomography
- Outcome of Primary Endocrine Therapy in Postmenopausal Patients with Endocrine-responsive Breast Cancer
- A Study on Skin-Sparing Mastectomy in Patients Ineligible for Breast-Conserving Therapy
- Three-Dimensional (3D) Imaging of Tumor Angiogenesis and its Inhibition : Evaluation of Tumor Vascular-Targeting Agent Efficacy in the DMBA-induced Rat Breast Cancer Model by Confocal Laser Scanning Microscopy (CLSM)
- Isolation and Characterization of a Novel Acid Proteinase, Tropiase from Candida tropicalis IFO 0589
- Isolation and Characterization of a Novel Elastase Inhibitor, AFLEI from Aspergillus flavus
- Esophageal Cancer Surgery in Elderly Patients 80 Years of Age or Older
- Spontaneous Rupture of the Intrahepatic Bile Duct due to Carcinoma of the Ampulla of Vater: A Case Report
- A Case of Obstructive Cholangitis of the Accessory Hepatic Duct Occurring 30 years after a Cholecystectomy
- Isolation and Characterization of a β-Primeverosidase Concerned with Alcoholic Aroma Formation in Tea Leaves
- Ultrastructural Changes in Mouse Leydig Cells after Streptozotocin Administration
- Curative Resection after a Response to Treatment with mFOLFOX6 for Unresectable Sigmoid Colon Cancer: Report of a Case
- Biological properties of elastase inhibitor, AFLEI from Aspergillus flavus
- The Effect of an Aspergillus fumigatus Derived Elastolytic Proteinase on Human Neutrophils
- Characterization of Elastolytic Proteinase from Aspergillus flavus:Comparison of Biochemical Properties to the Elastolytic Proteinase from Aspergillus fumigatus
- Isolation and Characterization of Elastolytic Proteinase from Aspergillus fumigatus
- Mature and activated osteoclasts exist in the synovium of rapidly destructive coxarthrosis
- Effects of glucocorticoid on adipocyte size in human bone marrow
- A simple method to determine the pelvic inclination angle based on anteroposterior radiographs
- ラット卵巣におけるインヒビンおよびアクチビンα,βAおよびβBサブユニットの局在〔英文〕
- Effects of a Prescription of Chinese Herbal Medicine on Snake Venom-Induced Nephropathy in Mice
- 百歩蛇毒投与によるマウスの糸球体障害に対する草薬処方剤の抑制効果についての病理学的検討〔英文〕
- Unresectable Advanced Esophageal Cancer Responded to Docetaxel/nedaplatin/5-fluorouracil Therapy: A Case Report
- Experimental Tracheal Replacement Using the Esophagus and an Expandable Metallic Stent
- A Case of Vogt-Koyanagi-Harada Disease Associated with Malignant Lymphoma
- Ureterocolic Fistula Secondary to Colonic Diverticulitis
- High-yields heterologous production of the novel Aspergillus fumigatus elastase inhibitor AFUEI in Aspergillus oryzae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Immunoreactive βA Subunit of Inhibin/Activin Is Present in Cytoplasm of Rat Spermatogenic Cells
- Characterization and Primary Structure of Elastase Inhibitor, AFLEI from Aspergillus flavus
- Clinical Experience with Smart Care for Respiratory Managementafter Esophageal Cancer Surgery
- Abdominal lymph node metastasis from prostatic adenocarcinoma in a patient with esophageal squamous cell carcinoma
- Acquired Immune-deficiency Syndrome with Focal Onset of Mycobacterium avium Infection Displaying a Histological/Genetic Pattern of Disseminated Mycobacteria
- An Experimental Study on Lipid Metabolism in Rats with Obstructive Jaundice ; Focusing on Cholesterol Synthesis
- Esophageal Duplication Cyst with Abscessation: A case report