Isolation and Characterization of a Novel Acid Proteinase, Tropiase from Candida tropicalis IFO 0589
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概要
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A novel acid proteinase (Tropiase) was isolated from Candida tropicalis IFO 0589 by DE52-cellulose, and DEAE-Cosmogel column chromatographies. The purified tropiase gave a single band on disc polyacrylamide gel electrophoresis, isoelectric focusing and sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzyme preparation had a molecular weight of 23,900, isoelectric point of pH 5.1, optimum pH range of 7 to 9 and possessed 208 amino acid residues. The enzyme hydrolyzed casein, fibrinogen, keratin and collagen. The purified tropiase demonstrated hemorrhagic and capillary permeability-increasing activities. Inhibition of tropiase occurred with leupeptin and N-bromosuccinimide, however, no inhibition was observed with α2 -macroglobulin, soybean trypsin inhibitor, benzamidine-HCl or diisopropyl fluorophosphate.
著者
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Nikai Toshiaki
Department Of Microbiology Faculty Of Pharmacy Meijo University
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Okumura Yoshiyuki
Department Of Validation Matsuurayakugyo Co. Ltd
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Inoue Naomasa
Scientific Affairs & Sales Promotion Group, Asahi Kasei Co., Ltd.
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Okumura Yoshiyuki
Department of Quality Control, Mathuurayakugyo Co., Ltd.
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Inoue Naomasa
Scientific Affairs & Sales Promotion Group, Asahi Kasei Co., Ltd.
関連論文
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