High-yields heterologous production of the novel Aspergillus fumigatus elastase inhibitor AFUEI in Aspergillus oryzae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
AFUEI, an elastase inhibitor produced by Aspergillus fumigatus strongly inhibits the elastolytic activity of A. fumigatus etc. To purify AFUEI, we constructed a strain that overproduces AFUEI by introducing the gene encoding AFUEI (Genbank accession no. AB546725) under control of the amyB promoter into the heterologous host Aspergillus oryzae. A. oryzae TF-4 displayed strong elastase inhibitory activity and produced considerably more AFUEI than that of A. fumigatus. Furthermore, AFUEI could be purified using culture broth and single ultrafiltration (UF) treatment, allowing for the effective production of AFUEI for use in clinical trials.
著者
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Nishimura Akira
Research And Development Department Hakutsuru Sake Brewing Co. Ltd.
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Ogawa Kenji
Department Of Pulmonary Medicine Higashi Nagoya National Hospital
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OKUMURA Yoshiyuki
Department of Validation, Matsuurayakugyo Co., Ltd.
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NIKAI Toshiaki
Department of Microbiology, Faculty of Pharmacy, Meijo University
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Ogawa Kenji
Department Of Orthopaedic Surgery Faculty Of Medicine Saga University
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MATSUI Takeshi
Department of Biochemical Engineering and Science, Kyushu Institute of Technology
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Komori Yumiko
Department Of Microbiology Faculty Of Pharmacy Meijo University
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Nikai Toshiaki
Department Of Microbiology Faculty Of Pharmacy Meijo University
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Uchiya Kei-ichi
Department Of Microbiology Faculty Of Pharmacy Meijo University
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Yamashita Nobuo
Research & Development Department Hakutsuru Sake Brewing Co. Ltd.
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Okumura Yoshiyuki
Department Of Validation Matsuurayakugyo Co. Ltd
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Nishimura Akira
Research & Development Department Hakutsuru Sake Brewing Co. Ltd
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Yamashita Nobuo
Research & Development Department Hakutsuru Sake Brewing Co. Ltd
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Ogawa Kenji
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Matsui Takeshi
Department Of Microbiology Faculty Of Pharmacy Meijo University
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Matsui Takeshi
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Okumura Yoshiyuki
Department of Quality Control, Mathuurayakugyo Co., Ltd.
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