Construction of a thiamine pyrophosphate high-producing strain of Aspergillus oryzae by overexpression of three genes involved in thiamine biosynthesis(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
We have found a gene (thiP) encoding thiamine pyrophosphokinase (TPK) in the Aspergillus oryzae genome. No riboswitch-like region was found in the upstream region of thiP, although it was repressed probably by thiamine pyrophosphate (TPP) as well as thiA and nmtA, which are strictly regulated by TPP-riboswitch sequence. To improve the productivity of TPP in A. oryzae, we constructed the strain in which thiA, nmtA and thiP were overexpressed simultaneously. The resulting strain accumulated intracellular TPP 4-fold higher than did the control strain.
- 公益社団法人日本生物工学会の論文
著者
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GOMI Katsuya
Laboratory of Bioindustrial Genomics, Graduate School of Agricultural Science, Tohoku University
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Nishimura Akira
Research And Development Department Hakutsuru Sake Brewing Co. Ltd.
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KUBODERA TAKAFUMI
Research and Development Department, Hakutsuru Sake Brewing Co., Ltd.
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YAMASHITA NOBUO
Research and Development Department, Hakutsuru Sake Brewing Co., Ltd.
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Yamashita Nobuo
Research And Development Department Hakutsuru Sake Brewing Co. Ltd.
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Nishimura A
Research And Development Department Hakutsuru Sake Brewing Co. Ltd.
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Gomi Katsuya
Laboratory Of Bioindustrial Genomics Department Of Bioindustrial Informatics And Genomics Graduate S
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Yamashita Nobuo
Research & Development Department Hakutsuru Sake Brewing Co. Ltd.
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Gomi Katsuya
Laboratory Of Bioindustrial Genomics Department Of Bioindustrial Informatics And Genomics Graduate S
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Kubodera Takafumi
Research And Development Department Hakutsuru Sake Brewing Co. Ltd.
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Tokui Misato
Research and Development Department, Hakutsuru Sake Brewing Co. Ltd.
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Tokui Misato
Research And Development Department Hakutsuru Sake Brewing Co. Ltd.
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Nishimura Akira
Research & Development Department Hakutsuru Sake Brewing Co. Ltd
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Yamashita Nobuo
Research & Development Department Hakutsuru Sake Brewing Co. Ltd
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Gomi Katsuya
Laboratory Of Bioindustrial Cenomics Graduate School Of Agricultural Science Tohoku University
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