Alternative Processing of Proproteins in Aspergilli kexB Gene Disruptants under Hyperosmotic Conditions

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概要

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• Disruption of the kexB gene encoding a subtilisin-like processing protease in Aspergillus oryzae and Aspergillus nidulans led to remarkable morphological defects, and these phenotypes were suppressed under hyperosmotic conditions. In this study, we investigated to determine whether non-KexB proteases might complement the in vivo function of KexB in the two Aspergillus kexB disruptants. Neither overexpression of opsA or opsB encoding A. oryzae aspartyl proteases homologous to yeast yapsins (YPS1/2) suppressed the kexB mutation, although yapsins are multicopy suppressors for the yeast kex2 mutation. A. nidulans and A. oryzae kexB disruptants grown under hyperosmotic conditions processed a recombinant fusion protein carrying a synthetic dibasic processing site (Lys-Arg) although the disruptants grown under normal growth conditions did not cleave the site. These results suggest that the two Aspergilli have other potential processing proteases that are induced and/or activated under hyperosmotic conditions and consequently complement, at least in part, the in vivo function of KexB.

• 社団法人 日本農芸化学会の論文

著者

• GOMI Katsuya

  Laboratory of Bioindustrial Genomics, Graduate School of Agricultural Science, Tohoku University

• FURUKAWA Kentaro

  Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University

• ABE Keietsu

  Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University

• FUJIOKA Tomonori

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• MIZUTANI Osamu

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• ICHIYANAGI Shunsuke

  Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural

• MATSUDA Yoshihiko

  Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural

• TOKUOKA Masafumi

  Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics, Graduate

• YAMAGATA Youhei

  Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural

関連論文

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• The β-1,3-Exoglucanase Gene exgA (exg1) of Aspergillus oryzae Is Required to Catabolize Extracellular Glucan, and Is Induced in Growth on a Solid Surface
• Alternative Processing of Proproteins in Aspergilli kexB Gene Disruptants under Hyperosmotic Conditions
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