Identification of N-terminal Autodigestion Target Site in Subtilisin ALP I
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概要
- 論文の詳細を見る
Autodigestion of subtilisin ALP I (ALP I), secreted from the alkalophilic Bacillus sp. NKS-21 and its predicted amino acid sequence having about 60% identity with other alkaline subtilisins, was examined under alkaline conditions. At the alkaline pH of 12,ALP I was rapidly degraded, and almost no breakdown products were detectable. However, by incubating ALP I at 5℃ for an extended time, a couple of specific peptides (26.7 kDa and 25.6 kDa) were accumulated. Each of them was purified and amino acid sequences of these fragments were found. Both peptides appeared to start at Gly-19 of the mature sequence of ALP I.
- 社団法人日本農芸化学会の論文
- 2001-05-23
著者
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NAKAJIMA Tasuku
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural
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MAEDA Hiroshi
Laboratory of Microbiology and Oncology, Faculty of Pharmaceutical Sciences, Sojo University
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Maeda H
Tsukuba R&d Center Fuji Oil Co Ltd.
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ICHISHIMA EIJI
Department of Bioengineering, Graduate School of Engineering, Soka University
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Ichishima Eiji
Department Of Bioengineering Graduate School Of Engineering Soka University
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Ichishima E
Department Of Bioengineering Graduate School Of Engineering Soka University
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Ichishima Eiji
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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YAMAGATA Youhei
Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
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Nakajima T
Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University
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YAMAGATA Youhei
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural
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Nakajima Tasuku
Laboratory Of Enzymology Division Of Life Science Graduate School Of Agricultural Science Tohoku Uni
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MAEDA Hiroshi
Production Engineering Research Laboratory, Matsushita Electric Works, Ltd.
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Maeda H
Fukuoka Inst. Of Technol. Fukuoka‐shi Jpn
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Yamagata Yukari
Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University
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Yamagata Y
Tohoku Univ. Sendai Jpn
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Yamagata Youhei
Dep. Of Applied Molecular Biology And Biochemistry Tokyo Univ. Of Agriculture And Technol.
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Yamagata Youhei
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
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Ichikawa Eiji
Gekkeikan Research Institute Gekkeikan Sake Co. Ltd.
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Yamagata Youhei
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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Nakajima Tasuku
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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Maeda Hiroshi
Laboratory Of Microbiology And Oncology Faculty Of Pharmaceutical Sciences Sojo University
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