Fluorescent Labeling of Sugar-carboxylate with Water-soluble Carbodiimide
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概要
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Fluorescent labeling of uronic acid with water-soluble carbodiimide, EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide), was examined. The optimum concentration of EDC was 100-200 mM and pH 4.75 was essential for the EDC activation of glucuronic acid. To form the amide linkage between the activated intermediate forms and a nucleophilic amino compound, a fluorescent reagent EDAN (N-1-ethylene-diaminonaphthalene) was used. Column chromatography with Bio-Gel P-2 was effective to remove excess EDAN from the fluorescent EDAN derivative of glucuronic acid. About 0.62-4.3μg/ml of glucuronic acid derivative gave a linear correlation with the relative fluorescence intensity. Although this method could be applicable to various lactones, mutual separation of these fluorescent derivatives by high performance liquid chromatography requires further examination.
- 社団法人日本農芸化学会の論文
- 1992-02-23
著者
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Ichishima Eiji
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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KOBAYASHI Mikihiko
National Food Research Institute
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