Characterization of the Complex between α_2-Macroglobulin and a Serine Proteinase from Bacillus natto(Biological Chemistry)

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概要

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• The stoichiometry of the reaction of bovine α_2-macroglobulin (α_2M) with a serine proteinase from Bacillus natto (BSP) showed that BSP bound to α_2M in a molar ratio of about 2:1. The α_2M-BSP complex hydrolyzed casein and succinyl-L-leucyl-L-leucyl-L-valyl-L-tyrosyl-4-methylcoumarin-7-am-ide (Suc-LLVY-MCA) 65 and 55% less, respectively, while the activity of fibrin hydrolysis was completely lost. The purified complex from DEAE-Toyopearl 650 S and Toyopearl HW-60 F chromatographies was homogeneous on polyacrylamide gel electrophoresis at pH 9.4. Immunoelectrophoresis of the α_2M-BSP complex showed weak cross-reactivity with an antiserum to BSP (anti-BSP). The specificity of α_2M-BSP complex to the acyl-LSTR-amide substrates Boc-LSTR-MCA and Boc-LSTR-pNA was partially changed. Enzymatic activity of the complex was completely abolished by chymostatin. Inactivation was observed when α_2M-BSP complex was incubated with a 5-molar excess of Streptomyces subtilisin inhibitor (SSI) at pH 7.5 and 20℃. Interaction of α_2M-BSP with ^<14>C-succinyl-SSI was done at 20℃ and at 4℃. Incorporation of ^<14>C-succinyl-SSI with α_2M-BSP was faster at 20℃ than 4℃.

• 社団法人日本農芸化学会の論文
• 1989-10-23

著者

• Nakamura Takashi

  Department of Cardiology, Saiseikai Shigaken Hospital

• ODA Kohei

  Department of Applied Biology, Kyoto Institute of Technology

• ICHISHIMA EIJI

  Department of Bioengineering, Graduate School of Engineering, Soka University

• NAKAJIMA TASUKU

  Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

• Ichishima Eiji

  Department Of Bioengineering Graduate School Of Engineering Soka University

• Ichishima E

  Department Of Bioengineering Graduate School Of Engineering Soka University

• Ichishima Eiji

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Murao Sawao

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• Murao S

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• YAMAGATA Youhei

  Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology

• Nakajima T

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• Oda K

  National Research Inst. Brewing Tokyo Jpn

• Oda Kohei

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• Nakajima Tasuku

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Murao Sawao

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture

• KAMATA Hirotoshi

  Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

• Yamagata Yukari

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• Yamagata Y

  Tohoku Univ. Sendai Jpn

• Yamagata Youhei

  Dep. Of Applied Molecular Biology And Biochemistry Tokyo Univ. Of Agriculture And Technol.

• Yamagata Youhei

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

• Yamagata Youhei

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Ichikawa Eiji

  Gekkeikan Research Institute Gekkeikan Sake Co. Ltd.

• Kamata Hirotoshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Nakamura Takashi

  Department Of Cardiology Saiseikai Shiga Hospital

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