IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF HUMAN T-CELL LEUKEMIA VIRUS TYPE-I ANTIGENS (<Symposium II>Immuno-Histochemistry and Cytochemistry)

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The location of human T-cell lenkemia virus type I (HTLV-I) antigens in HTLV-I-producing cells, HUT 102 and MT-2, was described on the basis of immunoelectron microscopic studies and immunoblotting analysis. The direct and indirect peroxidase-labeled antibody methods with monoclonal and polyclonal antibodies to various HTLV-I antigens as well as sera from patient with adult T-cell leukemia were used for the immunoelectron microscopic observation. In HTLV-I-producing cells fixed with paraformaldehyde and incubated in cell suspension with sera from ATL patients, the viral envelope and plasma membrane are positively stained. In the specimens frozen-sectioned and incubated with the patient sera after fixation, positive staining is observed mainly on the viral envelope, plasma membrane, nuclear envelope, and endoplasmic reticulum of thesc fells, gag gene product p24 was localized in the viral corc, and p19 was in the viral envelope and core and some parts of the plasma membrane. env gene product gp46^x was deduced to be localized on the viral envelope and the plasma membrane, and env precursor protcin gp62^x was ddduced to be in the endoplasmic reticulum and the nuclear envelope, pX gene product p40^x was localized mainly in the euchromatin regions of the nuclei of HTLV-I-producing cells, but p68^x of MT-2 cells was localized maumly in the nuclear envelope and the endoplasmic reticulum. The p68^x detected in MT-2 cells with anti-p40^x serum was deduced to be a fused protein consisting of a part of pX and env gene products and to share epitopes with p40^x.
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IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF HUMAN T-CELL LEUKEMIA VIRUS TYPE-I ANTIGENS (<Symposium II>Immuno-Histochemistry and Cytochemistry)

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