Glucan Binding Regions of Dextransucrase from Leuconostoc mesenteroides NRRL B-512F
スポンサーリンク
概要
- 論文の詳細を見る
We isolated glucan-binding peptides of a dextransucrase from Leuconostoc mesenteroides B-512F. The dextransucrase was bound to DEAE-Sephadex A-50, Sephadex G-100, and mutan from Streptococcus mutans. Mild trypsin digestion dissociated the enzyme and glucan binding. In the presence of ammonium sulfate, several peptides were bound to glucan after trypsin digestion. Four main mutan-binding peptides were obtained by this method, and those amino acid sequences were analyzed. One of them was identical with the dextran-binding peptide that contains lysine, which was previously isolated by differential chemical modification with o-phthalaldehyde. We also found mutan-binding peptides in sucrose- and dextran-binding regions and a lysine-rich region. Also, there was a peptide similar in sequence to glucan-binding A-repeat of streptococcal glucosyltransferases.
- 社団法人日本農芸化学会の論文
- 1998-01-23
著者
-
Ookura Tetsuya
National Food Res. Inst. Ibaraki
-
KOBAYASHI Mikihiko
National Food Research Institute
-
FUNANE Kazumi
National Food Research Institute
-
OOKUBO Tetsuya
National Food Research Institute
関連論文
- Primary Structure Analysis and Functional Expression of Erythrose Reductases from Erythritol-Producing Fungi (Trichosporonoides megachiliensis SNG-42)
- Protein Disulfide Isomerase Activity of Some Plant Seeds
- Pyrophosphate-dependent Phosphofructokinase : Its Oligomeric Forms in Mature Green Tomato Fruit
- Mold Pectinase Modified with Dialdehyde Derivatives of Dextran and Cellulose
- Saccharification of Beet Pulp and Its Reduced Derivatives
- Sugar Composition of Beet Pulp Polysaccharides and Their Enzymatic Hydrolysis
- Aggregated Form of Dextransucrases from Leuconostoc mesenteroides NRRL B-512F and Its Constitutive Mutant
- Condensation of Dextran-dialdehyde with Amino Acids under Nonreductive Conditions
- Protein Kinase Activity in the Prokaryote, Micrococcus luteus FK1001(Biological Chemistry)
- Fluorescent Activity Assay of the Enzymes Hydrolyzing Acidic Polysaccharides
- Reduction of Allergenic Proteins by the Effect of the ripening inhibitor (rin) Mutant Gene in an F_1 Hybrid of the rin Mutant Tomato
- Preparation of new PTCR material by particle composition
- An arrangement of micrometer-sized powder particles by electron beam drawing
- Identification of Catalytic Amino Acids of Cyclodextran Glucanotransferase from Bacillus circulans T-3040
- Construction of Chimeric Glucansucrases for Analyzing Substrate-binding Regions That Affect the Structure of Glucan Products
- Manipulation of fine objects by probes of the electrostatic chuck type
- Application of probe manipulation to the fabrication of a smart probe
- Purification of Amylases and Other Enzymes by a Forced-affinity Chromatography Method
- Identification and Characterization of a Family of Genes for the Plasma Membrane H^+ -ATPase of Oryza sativa L. : GENES STRUCTURE AND EXPRESSION : MEMBRANES AND BIOENERGETICS
- Isolation of Bacillus and Paenibacillus Bacterial Strains That Produce Large Molecules of Cyclic Isomaltooligosaccharides
- Isolation of Bacillus and Paenibacillus Bacterial Strains That Produce Large Molecules of Cyclic Isomaltooligosaccharides
- Comparison of o-Phthalaldehyde Modification of α-Amylases from Porcine Pancreas and Bacillus subtilis with Taka-amylase A
- Flutolanil Resistance as a Genetic Marker of Coprinus cinereus Strains
- Cloning and Sequence Analysis of the Gene for Glucodextranase from Arthrobacter globiformis T-3044 and Expression in Escherichia coli Cells
- Glucan Binding Regions of Dextransucrase from Leuconostoc mesenteroides NRRL B-512F
- Novel Cyclic Dextrins, Cycloisomaltooligosaccharides, from Bacillus sp. T-3040 Culture
- Fluorescent Labeling of Sugar-carboxylate with Water-soluble Carbodiimide
- Chemical Modification of Potato Phosphorylase by o-Phthalaldehyde
- Substrate Binding Ability of Chemically Inactivated Pectinase for the Substrate Pectic Acid
- A Model System for Convenient Fluorescent Labeling of Sugar Chain in Taka-amylase A
- Isolation of Taka-amylase A Peptides Required for Substrate Binding
- Electrophoretic Analysis of Chemically Modified Taka-amylase A Using a Slab Gel System with Multiple Lanes
- Inhibition of Dextran and Mutan Synthesis by Cycloisomaltooligosaccharides
- Characterization of Iron (II) : Comoplexes with β-Diketones and Monthio-β-diketone (Commemoration Issue Dedicated to Professor Tsunenobu Shigematsu on the Occasion of his Retirement)