Construction of Chimeric Glucansucrases for Analyzing Substrate-binding Regions That Affect the Structure of Glucan Products
スポンサーリンク
概要
- 論文の詳細を見る
A gene that encodes dextransucrase S (dsrS) from Leuconostoc mesenteroides NRRL B-512F encodes a glucansucrase dextransucrase S (DSRS) which mainly produces water-soluble glucan (dextran), while the dsrT5 gene derived from dsrT of the B-512F strain encodes an enzyme dextransucrase T5 (DSRT5), which mainly produces water-insoluble glucan. Tyr340-Asn510 of DSRS and Tyr307-Asn477 of DSRT5 (Site 1), Lys696-Gly768 of DSRS and Lys668-Gly740 of DSRT5 (Site 2), and Asn917-Lys1131 of DSRS and Asn904-Lys1118 of DSRT5 (Site 3) were exchanged and six different chimeric enzymes were constructed. Water-soluble glucan produced by recombinant DSRS was composed of 64% 6-linked glucopyranoside (Glcp), 9% 3,6-linked Glcp, and 13% 4-linked Glcp. Water-insoluble glucan produced by recombinant DSRT5 was composed of 47% 6-linked Glcp and 43% 3-linked Glcp. All of the chimeric enzymes produced glucans different from the ones produced by their parental enzymes. Some of the glucans produced by chimeric enzymes were extremely changed. The Site 1 chimeric enzyme of DSRS (STS1) produced water-soluble glucan composed mostly of 6-linked Glcp. That of DSRT5 (TST1) produced water-insoluble glucan composed mostly of 4-linked Glcp. The Site 3 chimeric enzyme of DSRS (STS3) produced mainly water-insoluble glucan, DSRT5 (TST3) produced mainly water-soluble glucans, and all of the glucan fractions consisted of 3-Glcp, 4-Glcp, and 6-Glcp. The amounts of the three linkages in the water-soluble glucan produced by TST3 were about 1:1:1. Site 1 was assumed to be important for making or avoiding making α-1,4 linkages, while Site 3 was assumed to be important for determining the kinds of glucosyl linkages made.
- 社団法人 日本農芸化学会の論文
- 2004-09-23
著者
-
KOBAYASHI Mikihiko
National Food Research Institute
-
FUNANE Kazumi
National Food Research Institute
-
Yamamoto Tomoko
National Food Research Institute
-
Kobayashi M
Biophotonics Information Laboratories Yamagata Advanced Technology Research And Development Center
-
Kobayashi Mikihiko
Jissen Women's Univ.
-
Kobayashi Mikihiko
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
-
TERASAWA Kazue
National Food Research Institute
-
ISHII Tadashi
Wood Biochemistry Forestry and Forest Products Research Institute
-
KOBAYASHI Masaki
Yamagata Advanced Technology Research and Development Center
-
Funane K
National Food Research Institute
関連論文
- Pyrophosphate-dependent Phosphofructokinase : Its Oligomeric Forms in Mature Green Tomato Fruit
- Mold Pectinase Modified with Dialdehyde Derivatives of Dextran and Cellulose
- Saccharification of Beet Pulp and Its Reduced Derivatives
- Sugar Composition of Beet Pulp Polysaccharides and Their Enzymatic Hydrolysis
- Modification of Potato and Yeast Phosphorylases with Cyclodextrin-Dialdehyde(Biological Chemistry)
- Affinity Labeling of Muscle Phosphorylase b with α-Cyclodextrin-Dialdehyde(Biological Chemistry)
- Inhibition of α-Amylase and Phosphorylases by Cyclodextrin-Dialdehyde(Biological Chemistry)
- Cyclodextrin-Dialdehyde Prepared by Periodate Oxidation(Biological Chemistry)
- Characterization of an Isoform of Rice Starch Branching Enzyme, RBE4, in Developing Seeds
- Gene Encoding a Dextransucrase-like Protein in Leuconostoc mesenteroides NRRL B-512F
- Aggregated Form of Dextransucrases from Leuconostoc mesenteroides NRRL B-512F and Its Constitutive Mutant
- Condensation of Dextran-dialdehyde with Amino Acids under Nonreductive Conditions
- Development and applications of new technology for two-dimensional space-time characterization and correlation analysis of ultraweak biophoton information
- Fluorescent Derivatives of Polysaccharide Dialdehyde as Substrates for Glucanases(Biological Chemistry)
- Highly Reactive Dialdehydes of Cellulose and α-Cyclodextrin(Biological Chemistry)
- Low-level Chemiluminescence of Water-imbibed Soybean Seeds(Biological Chemistry)
- Preparation and Characterization of Taka-amylase A Attached to Carboxymethyl Dextran by Water-soluble Carbodiimide(Biological Chemistry)
- Use of Water-soluble Carbodiimide(EDC) for Immobilization of EDC-sensitive Dextranase(Biological Chemistry)
- Yeast Glycogen Phosphorylase: Kinetic Properties Compared with Muscle and Potato Enzymes(Biological Chemistry)
- Purification and Some Properties of Two Wall-associated (β1,3)Glucanases from Neurospora crassa Cells(Biological Chemistry)
- Human Renin-Binding Protein Is the Enzyme N-Acetyl-D-Glucosamine-2-Epimerase
- Protein Kinase Activity in the Prokaryote, Micrococcus luteus FK1001(Biological Chemistry)
- Fluorescent Activity Assay of the Enzymes Hydrolyzing Acidic Polysaccharides
- Preparation of new PTCR material by particle composition
- An arrangement of micrometer-sized powder particles by electron beam drawing
- Identification of Catalytic Amino Acids of Cyclodextran Glucanotransferase from Bacillus circulans T-3040
- Construction of Chimeric Glucansucrases for Analyzing Substrate-binding Regions That Affect the Structure of Glucan Products
- Manipulation of fine objects by probes of the electrostatic chuck type
- Application of probe manipulation to the fabrication of a smart probe
- Identification of Cysteine-380 as the Essential Residue for the Human N-Acetyl-D-Glucosamine 2-Epimerase (Renin Binding Protein)
- Changes in Starch Content and Related Enzyme Activities during the Growth of Germinating Soybeans
- Purification of Amylases and Other Enzymes by a Forced-affinity Chromatography Method
- In vivo imaging of spontaneous ultraweak photon emission from a rat's brain correlated with cerebral energy metabolism and oxidative stress
- Substrate Binding Sites of Leuconostoc Dextransucrase Evaluated by Inhibition Kinetics(Biological Chemistry)
- Isolation of Bacillus and Paenibacillus Bacterial Strains That Produce Large Molecules of Cyclic Isomaltooligosaccharides
- Isolation of Bacillus and Paenibacillus Bacterial Strains That Produce Large Molecules of Cyclic Isomaltooligosaccharides
- Comparison of o-Phthalaldehyde Modification of α-Amylases from Porcine Pancreas and Bacillus subtilis with Taka-amylase A
- Cloning and Sequence Analysis of the Gene for Glucodextranase from Arthrobacter globiformis T-3044 and Expression in Escherichia coli Cells
- Glucan Binding Regions of Dextransucrase from Leuconostoc mesenteroides NRRL B-512F
- Novel Cyclic Dextrins, Cycloisomaltooligosaccharides, from Bacillus sp. T-3040 Culture
- Fluorescent Labeling of Sugar-carboxylate with Water-soluble Carbodiimide
- Chemical Modification of Potato Phosphorylase by o-Phthalaldehyde
- Substrate Binding Ability of Chemically Inactivated Pectinase for the Substrate Pectic Acid
- A Model System for Convenient Fluorescent Labeling of Sugar Chain in Taka-amylase A
- Isolation of Taka-amylase A Peptides Required for Substrate Binding
- Two Phosphorylases from Suspension-cultured Soybeans(Biological Chemistry)
- Electrophoretic Analysis of Chemically Modified Taka-amylase A Using a Slab Gel System with Multiple Lanes
- Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans
- Inhibition of Dextran and Mutan Synthesis by Cycloisomaltooligosaccharides
- Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity.
- An Isomaltotriose-producing Dextranase from Flavobacterium sp. M-73: Purification and Properties
- Intensive UV Absorption of Dextrans and Its Application to Enzyme Reactions
- Fluorospectral Intensity of High-molecular-weight Dextrans: Application to the Enzyme Reactions
- Activation of Dextransucrase from Leuconostoc mesenteroides by the Substrate, Dextran
- Comparison of the Multiplicity of Dextransucrases from Six Strains of Leuconostoc mesenteroides
- Structural Characteristics of Water-soluble Dextran from Leuconostoc mesenteraides NRRL B-1298
- Effectors Differently Modulating the Dextransucrase Activity of Leuconostoc mesenteroides
- Yeast Glycogen Phosphorylase: Characterization of the Dimeric Form and Its Activation
- Identification of Cysteine-380 as the Essential Residue for the Human N-Acetyl-D-Glucosamine 2-Epimerase (Renin Binding Protein).
- Characterization of Iron (II) : Comoplexes with β-Diketones and Monthio-β-diketone (Commemoration Issue Dedicated to Professor Tsunenobu Shigematsu on the Occasion of his Retirement)