Affinity Labeling of Muscle Phosphorylase b with α-Cyclodextrin-Dialdehyde(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Rabbit muscle phosphorylase b was modified with a substrate analog, the 2',3'-dialdehyde derivative of α-cyclodextrin (dial-α-CD). Although the inhibition of phosphorylase b by α-,β-, and γ-cyclodextrins gave rather high Ki values (10〜25mM), the dial-CD gave much smaller Ki values of 1.2〜3.5mM. Moreover, the latter inhibition was time-dependent and accelerated by higher pHs and higher concentrations fo dial-CD. Incorporation of the dial-CD into the enzyme was proportional to the loss of enzyme activity and became stationary at about I mol of dial-CD bound toa mol of enzyme subunit. Modification was greatly suppressed by the presence of substrate glycogen. Glucose 1-phosphate was not effective. The dial-CD-modified phosphorylase b was purified by Sephadex G-75 and Con A-Sepharose column chromatography. The modified enzyme gave a single band of activity having a K_<app> of 6.3% glycogen on affinity gel electrophoresis, which showed that the modified enzyme had a very low affinity for glycogen. Comparison of Km values of the native and modified phosphorylase b showed that the Km values for the glucan substrates increased 8 to 11-fold in the modified enzyme. These results suggested that the glycogen storage site of muscle phosphorylase b might be modified with dial-CD and the modified enzyme significantly decreased in the affinity for the substrate glycogen.
- 社団法人日本農芸化学会の論文
- 1988-11-23
著者
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Takagi Shiro
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Tanabe Satoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Matsuda Kazuo
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University:(present Office)iwaki-
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Matsuda Kazuo
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Takagi S
Tohoku Univ. Sendai Jpn
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ICHISHIMA EIJI
Department of Bioengineering, Graduate School of Engineering, Soka University
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Ichishima Eiji
Department Of Bioengineering Graduate School Of Engineering Soka University
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Ichishima E
Department Of Bioengineering Graduate School Of Engineering Soka University
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Ichishima Eiji
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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KOBAYASHI Mikihiko
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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URAYAMA Tadanori
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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SUZAWA Itsuko
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Kobayashi M
Biophotonics Information Laboratories Yamagata Advanced Technology Research And Development Center
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Kobayashi Mikihiko
Jissen Women's Univ.
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Kobayashi Mikihiko
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Suzawa Itsuko
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Matsuda K
Iwaki Meisei Univ. Fukushima Jpn
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KOBAYASHI Masaki
Yamagata Advanced Technology Research and Development Center
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Ichikawa Eiji
Gekkeikan Research Institute Gekkeikan Sake Co. Ltd.
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Urayama Tadanori
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Matsuda Kazuo
Department Of Agricultural Biochemistry Faculty Of Agriculture Tohoku University
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