Purification and Some Properties of an Endo-β-1,6-glucanase from Neurospora crassa(Biological Chemistry)
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概要
- 論文の詳細を見る
An endo-β-1,6-glucanase (E.C. 3.2.1.75) was purified from the culture filtrate of Neurospora crassa IFO-6068 by chromatographies on CM-cellulofine, Con-A Sepharose 4B, and Sepharose CL-6B followed by preparative affinity gel electrophoresis. The purified enzyme had an apparent molecular weight of 47,000. The pH and temperature optima for the activity were 5.0 and 50℃. The enzyme acted on β-1,6-glucan (Pustulan) and yielded a series of gentio-oligosaccharides with endo-type action, and finally, glucose and gentiobiose were produced. The enzyme was also able to act on N. crassa cell wall β-glucan, and a small amount of hydrolysis fragments were liberated without apparent change of the cell wall glucan molecules.
- 社団法人日本農芸化学会の論文
- 1987-12-23
著者
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Matsuda Kazuo
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University:(present Office)iwaki-
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Matsuda Kazuo
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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NAKAJIMA TASUKU
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Nakajima T
Hoechst Japan Ltd. Saitama Jpn
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Nakajima Tasuku
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Matsuda K
Iwaki Meisei Univ. Fukushima Jpn
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Hiura Nozomi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Matsuda Kazuo
Department Of Agricultural Biochemistry Faculty Of Agriculture Tohoku University
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