Purification and Characterization of an Endo α-1,3-D-Mannanase from Flavobacterium sp. AS-9
スポンサーリンク
概要
- 論文の詳細を見る
A novel mannanase that has a strict specificity for the α-1,3-mannosidic linkage has been isolated and characterized. The enzyme was purified to homogeneity from the cultural supernatant of a soil bacterium, Flavobacterium sp. AS-9 isolated by enrichment culture on Auricularia mannan of which the structure was an α-1,3-linked D-mannan main chain branched with short segments of xylose or glucuronic acid. Purified mannanase was optimally active between pH 6 and 8. The apparent molecular mass of the enzyme was 94 kDa by SDS gel electrophoresis under reducing conditions and 100 kDa by gel filtration HPLC. The enzyme only hydrolyzed α-1,3-D-mannan with endo-type action to produce α-1,3-mannooligosaccharides of various sizes. The smallest product was α-1,3-mannobiose.
- 社団法人日本農芸化学会の論文
- 1996-10-23
著者
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Maruyama Y
Tohoku Univ. Sendai Jpn
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Maruyama Yutaka
Department of Toxicology, School of Veterinary Medicine, Rakuno-Gakuen University
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ICHISHIMA EIJI
Department of Bioengineering, Graduate School of Engineering, Soka University
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NAKAJIMA TASUKU
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Ichishima Eiji
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Matsumoto T
Tohoku Univ. Sendai Jpn
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SATO Asako
Department of Applied Biological Chemistry, Tohoku University
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MATSUMOTO Takehiko
Department of Applied Biological Chemistry, Tohoku University
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SUENAGA Masahiro
Department of Applied Biological Chemistry, Tohoku University
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Maruyama Yutaka
Department Of Toxicology School Of Veterinary Medicine Rakuno-gakuen University
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Matsumoto Takehiko
Department Of Applied Biological Chemistry Tohoku University
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Nakajima Tasuku
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Sato Asako
Department Of Applied Biological Chemistry Tohoku University
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Ichishima Eiji
Department Of Applied Biological Chemistry Tohoku University
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Suenaga Masahiro
Department Of Applied Biological Chemistry Tohoku University
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NAKAJIMA Tasuku
Department of Applied Biological Chemistry, Tohoku University
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