Characterization of Chimeric Enzymes Constructed between Two Distinct α-Amylase cDNAs from Cultured Rice Cells
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概要
- 論文の詳細を見る
Cultured cells of rice (Oryza sativa cv Sasanishiki) produce two α-amylase isozymes, AMY-I and AMY-III. Using a bacterial expression system, eight chimeric genes constructed with various combination of AMY-I and AMY-III cDNA fragments were expressed, and each recombinant chimeric protein was characterized. Four of the either recombinant enzymes having region c (one of the four regions having unconserved base sequences between AMY-I and AMY-III cDMAs) of AMY-I showed the same enzyme characteristics as that of native AMY-I, which had high temperature optimum at 50℃. The other four chimeric proteins carring region c of AMY-III showed the AMY-III type characteristics, which were a low temperature optimum at 25℃ and susceptibility to a higher maltooligosaccharide (G17) substrate. The unconserved region c is involved in the decision of the characteristic of AMY-I or AMY-III.
- 社団法人日本農芸化学会の論文
- 1999-08-23
著者
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Aoyagi M
Univ. Tsukuba Ibaraki
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Ichishima Eiji
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Nakajima Tasuku
Laboratory Of Enzymology Division Of Life Science Graduate School Of Agricultural Science Tohoku Uni
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Kasahara S
Tohoku Univ. Sendai Jpn
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Kasahara Shin
Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University
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Ichishima Eiji
Department Of Bioengineering Faculty Of Engineering Soka University
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ABE Rei
Laboratory of Enzymology, Division of Life Science, Graduate School of Agricultural Science, Tohoku
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YOSHIDA Kensuke
Laboratory of Enzymology, Division of Life Science, Graduate School of Agricultural Science, Tohoku
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AOYAGI Masanobu
Laboratory of Enzymology, Division of Life Science, Graduate School of Agricultural Science, Tohoku
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Abe Rei
Laboratory Of Enzymology Division Of Life Science Graduate School Of Agricultural Science Tohoku Uni
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Yoshida Kensuke
Laboratory Of Enzymology Division Of Life Science Graduate School Of Agricultural Science Tohoku Uni
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Nakajima Tasuku
Laboratory Of Enzymology Division Of Life Science Graduate School Of Agricultural Science Tohoku Uni
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Nakajima Tasuku
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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