Functional Analysis of an Endo-1,6-β-D-glucanase Gene (neg-1) from Neurospora crassa

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概要

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• The 1,6-β-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.

• 社団法人 日本農芸化学会の論文
• 2006-07-23

著者

• Abe Kohji

  Laboratory Of Nutrition Chemistry

• Abe K

  Agricultural And Life Sciences Dep. Of Applied Biological Chemistry

• NAKAJIMA Tasuku

  Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural

• YAMAGATA Youhei

  Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology

• Nakajima T

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• YAMAGATA Youhei

  Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural

• Abe K

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• OYAMA Seiji

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• INOUE Hirokazu

  Laboratory of Genetics, Department of Regulation Biology, Faculty of Science, Saitama University

• ABE Keietu

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• Nakajima Tasuku

  Laboratory Of Enzymology Division Of Life Science Graduate School Of Agricultural Science Tohoku Uni

• Oyama Seiji

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• Inoue Hirokazu

  Laboratory Of Genetics Department Of Regulation Biology Faculty Of Science Saitama University

• Yamagata Yukari

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• Yamagata Y

  Tohoku Univ. Sendai Jpn

• Abe Keietsu

  Divison Of Applied Life Science Graduate School Of Agricultural Science Tohoku University

• Yamagata Youhei

  Dep. Of Applied Molecular Biology And Biochemistry Tokyo Univ. Of Agriculture And Technol.

• Yamagata Youhei

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

• Yamagata Youhei

  Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc

• Nakajima Tasuku

  Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc

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