Cloning and Expression of the Exo-β-D-1,3-glucanase Gene (exgS) from Aspergillus saitoi(Biochemistry & Molecular Biology)

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概要

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• A gene of exo-1,3-β-D-glucanase (exgS) was cloned from a koji mold, Aspergillus saitoi, genomic DNA using PCR. The exgS has an ORF comprising 2832 bp, which contains one intron of 45 bp, and encodes 945 amino acids. The deduced amino acid sequences showed that the ExgS has a non-homologous linker region consisting of 180 amino acids, which encompassed highly conserved regions observed in Exg homologues from filamentous fungi. A recombinant protein (ExgS) has been recovered from the cultural filtrate of an Aspergillus oryzae strain that carried an expression vector containing full length of the exgS. The N-terminal amino acid sequences of the recombinant exo-1,3-β-D-glucanase (ExgS) were identical to that of native ExgS from A. saitoi.

• 社団法人日本農芸化学会の論文
• 2002-07-23

著者

• Abe Kohji

  Laboratory Of Nutrition Chemistry

• Abe K

  Agricultural And Life Sciences Dep. Of Applied Biological Chemistry

• NAKAJIMA Tasuku

  Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural

• ABE Keietsu

  Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University

• YAMAGATA Youhei

  Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology

• Nakajima T

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• YAMAGATA Youhei

  Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural

• Abe K

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• ODA Ken

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• KASAHARA Shin

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• Nakajima Tasuku

  Laboratory Of Enzymology Division Of Life Science Graduate School Of Agricultural Science Tohoku Uni

• Kasahara Shin

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• Yamagata Yukari

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• Yamagata Y

  Tohoku Univ. Sendai Jpn

• Abe Keietsu

  Divison Of Applied Life Science Graduate School Of Agricultural Science Tohoku University

• Abe Keietsu

  Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc

• Yamagata Youhei

  Dep. Of Applied Molecular Biology And Biochemistry Tokyo Univ. Of Agriculture And Technol.

• Yamagata Youhei

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

• Oda Ken

  Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University

• Yamagata Youhei

  Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc

• Nakajima Tasuku

  Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc

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