The aman6 Gene Encoding a Yeast Mannan Backbone Degrading 1,6-α-D-Mannanase in Bacillus circulans : Cloning, Sequence Analysis, and Expression
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概要
- 論文の詳細を見る
A gene (aman6) encoding endo-1,6-α-D-mannanase, a yeast mannan backbone degrading enzyme from Bacillus circulans was cloned. The putative aman6 was 1767 base pairs long and encoded a mature 1,6-α-D-mannanase protein of 589 amino acids and a signal peptide of 36 amino acids. The purified mature 1,6-α-D-mannanase from the Escherichia coli transformant showed 61-kDa protein, and N-terminal amino acid sequence and other general properties of the recombinant enzyme were identical to those of 1,6-α-D-mannanase from Bacillus circulans TN-31.
- 社団法人日本農芸化学会の論文
- 2000-09-23
著者
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Maruyama Yutaka
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veteri
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NAKAJIMA Tasuku
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural
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Maruyama Yutaka
Laboratory Of Enzymology Division Of Life Science Graduate School Of Agricultural Science Tohoku Uni
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Nakajima Tasuku
Laboratory Of Enzymology Division Of Life Science Graduate School Of Agricultural Science Tohoku Uni
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Nakajima Tasuku
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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