Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the β-subunit of coatomer complex, β-COP
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概要
- 論文の詳細を見る
Calpain is a Ca2+-regulated cytosolic protease. Mammals have 14 calpain genes, half of which are predominantly expressed in specific organ(s); the rest are expressed ubiquitously. A defect in calpains causes lethality/pathogenicity, indicating their physiological indispensability. nCL-2/calpain-8a was identified as a stomach-specific calpain, whose physiological functions are unclear. To elucidate these, we characterized nCL-2 in detail. Unexpectedly, nCL-2 was localized strictly to the surface mucus cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum. Yeast two-hybrid screening identified several nCL-2-intracting molecules. Of these, the -subunit of coatomer complex (-COP) occurs in the stomach pit cells and is proteolyzed by nCL-2 in vitro. Furthermore, -COP and nCL-2 co-expressed in COS7 cells co-localized in the Golgi, and Ca2+-ionophore stimulation caused the proteolysis of -COP near the linker region, resulting in the dissociation of -COP from the Golgi. These results strongly suggest novel functions for nCL-2 that involve the membrane trafficking of mucus cells via interactions with coat protein.
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGYの論文
- 2006-04-21
著者
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Abe Kohji
Laboratory Of Nutrition Chemistry
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Suzuki Kazuharu
Department Of Nutritional Science Tokyo University Of Agriculture
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Suzuki Kazuharu
東京農業大学 農学部栄養科学科
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Abe K
Agricultural And Life Sciences Dep. Of Applied Biological Chemistry
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Abe Keiko
Agricultural And Life Sciences Dep. Of Applied Biological Chemistry
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Suzuki K
Univ. Tokyo Tokyo
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Suzuki K
Taisho Pharmaceutical Co. Ltd. Saitama Jpn
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Suzuki K
Department Of Nutritional Science Tokyo University Of Agriculture
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Abe K
Laboratory Of Enzymology Graduate School Of Agricultural Science Tohoku University
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Kitagaki Hiroshi
Department Of Biochemistry Meiji Pharmaceutical University
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Abe Keietsu
Divison Of Applied Life Science Graduate School Of Agricultural Science Tohoku University
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