Molecular Cloning and Sequence Analysis of the Gene Encoding the Collagenase from Cytophaga sp. L43-1 Strain
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概要
- 論文の詳細を見る
Cytophaga sp. strain L43-1 secretes a collagenase [Y. Sasagawa et al., Biosci. Biotech. Biochem., 57, 1894-1898 (1993)]. A cog gene encoding the collagenase from this strain was cloned, and the nucleotides were sequenced. The structural gene of cog consisted of 3846 base pairs, which encoded a polypeptide consisting of 1282 amino acid residues with a predicted molecular mass of 130kDa which was synthesized as a pre-matured enzyme. The deduced N-terminal 14 amino acids sequence, molecular mass of 120kDa, and pI of 4.96 of the predicted matured enzyme were consistent with those previously found for the collagenase purified from the strain. The cog gene was expressed in Escherichia coli using the lac promoter and ribosomal binding sequence in plasmid vector pUC119 or pKK223-3, but not its own putative promoter and Shine-Dalgarno sequence. The consensus amino acid sequence (His-Glu-Xaa-Xaa-His) of an active site of the metal proteases including the collagenase from Vibrio arginolyticus and a series of human MMPs was found in the Cog protein of the strain.
- 社団法人日本農芸化学会の論文
- 1995-11-23
著者
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Suzuki Kazuharu
東京農業大学 農学部栄養科学科
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Kojima H
Kanazawa Univ. Ishikawa Jpn
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IZAKI Kazuo
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Suzuki K
Univ. Tokyo Tokyo
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KAMIO Yoshiyuki
Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University
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Matsubara Yuko
Transgenic Animal Research Center National Institute Of Agrobiological Sciences
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Matsubara Yuko
Research Institute Of Nippi Incorporated
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Sasagawa Yoshikiyo
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University
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Suzuki Koki
Research Institute of Nippi, Incorporated
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Kojima Hisao
Research Institute of Nippi, Incorporated
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Kojima H
Special Division For Green Life Technology National Institute Of Advanced Industrial Science And Tec
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Izaki K
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Izaki Kazuo
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Kamio Yoshiyuki
Department Of Agricultural Chemistry Tohoku University
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Sasagawa Yoshikiyo
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Suzuki Kazuharu
Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture
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