Sequential Binding of Staphylococcal γ-Hemolysin to Human Erythrocytes and Complex Formation of the Hemolysin on the Cell Surface
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概要
- 論文の詳細を見る
Staphylococcal γ-hemolysin consists of two protein components, F (or HγI) and HγII. To elucidate the mode of action of γ-hemolysin, we studied the binding order of F and HγII to human erythrocytes and the cell-bound state of the two components. The binding of F to human erythrocytes preceded the binding of HγII to the cells, and thereafter hemolysis occurred. Western immunoblot analysis of the cell-bound γ-hemolysin indicated that F and HγII components form high-molecular-mass (150-250 kDa) complexes on the erythrocytes. The toxin complexes were recovered in a Triton X-100-insoluble fraction of the erythrocytes, which contains cytoskeleton proteins. Neither the formation of the toxin complex(es) nor hemolysis occurred when the erythrocytes were treated with proteinase K. Abortion of the complex formation on the proteinase K-treated erythrocytes may be due to the failure of the binding of HγII to the cells, because F bound to the proteinase K-treated erythrocytes to the same extent as to the non-treated erythrocytes.
- 社団法人日本農芸化学会の論文
- 1997-05-23
著者
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金子 淳
東北大学大学院農学研究科応用微生物学分野
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Kaneko Jun
Tohoku Univ. Sendai Jpn
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Ozawa T
Univ. Tsukuba Ibaraki Jpn
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OZAWA Toshiko
Department of Periodontics and Endodontics, Tsurumi University School of Dental Medicine
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金子 淳
東北大農化
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Kaneko Jun
Department Of Applied Microbiology Graduate School Of Agricultural Science Tohoku University
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TOMITA Toshio
Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University
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KAMIO Yoshiyuki
Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University
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Ozawa T
Tochigi Res. Lab. Kao Corp. Tochigi Jpn
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Tomita T
Showa Sangyo Co. Ltd.
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Tomita Toshio
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Ozawa Toshiko
Department Of Periodontics And Endodontics Tsurumi University School Of Dental Medicine
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Kamio Yoshiyuki
Department Of Agricultural Chemistry Tohoku University
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