Characterization of Trehalose Phosphorylase from Bacillus stearothermophilus SK-1 and Nucleotide Sequence of the Corresponding Gene(Biochemistry & Molecular Biology)

スポンサーリンク

概要

• 論文の詳細を見る

• A bacterial trehalose phosphorylase (TPase ; EC 2. 4. 1. 64) was purified from the culture supernatant of Bacillus stearothermophilus SK-1 to apparent homogeneity, and some properties were investigated. Furthermore, a gene from SK-1 responsible for the TPase was cloned by Southern hybridization with a degenerate oligonucleotide probe synthesized on the basis of the N-terminal sequence of the purified enzyme. The M_r of the enzyme was estimated to be 150,000 by gel filtration and 83,000 by SDS-PAGE, so the enzyme is likely to be a homodimer. The enzyme had optimum activity at pH 7.0-8.0 or nearby and the optimum temperature was about 75℃. The deduced amino acid sequence of the SK-1 TPase encodes a theoretical protein with a M_r of 87,950. Alignment of amino acid sequences with a maltose phosphorylase from Lactobacillus brevis the crystal structure and active site of which had been analyzed suggested that these two phosphorylases evolved from a common ancestor. The Escherichia coli cells harboring the plasmid containing the cloned TPase gene had about 100 times the activity of SK-1.

• 社団法人日本農芸化学会の論文
• 2002-09-23

著者

• Inoue Yuichi

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• 弥武 経也

  昭和産業(株)技術部

• Fukui Fumio

  Showa Sangyo Co. Ltd.

• Inoue Y

  Showa Sangyo Co. Ltd.

• Inoue Y

  Solar Energy Research Group Institute For Physical And Chemical Research

• Inoue Yorinao

  Photosynthesis Research Lab.

• INOUE Yasushi

  Showa Sangyo Co., Ltd.

• TOMITA Tetsuji

  Showa Sangyo Co., Ltd.

• YATAKE Tsuneya

  Showa Sangyo Co., Ltd.

• ISHII Keiko

  Showa Sangyo Co., Ltd.,

• Yoshino Ichiro

  The Department Of Surgery Ii Faculty Of Medicine University Of Occupational And Environmental Health

• Ishii Keiko

  Department Of Laboratory Medicine Shinshu University School Of Medicine Nagano

• Tomita T

  Showa Sangyo Co. Ltd.

• Yatake T

  Showa Sangyo Co. Ltd.

• Ishii Keiko

  Showa Sangyo Co. Ltd.

• Yatake Tsuneya

  Research And Development Center Showa Sangyo Co. Ltd.

• Yatake Tsuneya

  R & D Center Kashima Division Showa Sangyo Co. Ltd.

関連論文

• Cooperation of Photosynthetic Reaction Center of Photosystem II under Weak Light as Shown by Light Intensity-dependence of the Half-effective Dose of Atrazine
• Successful Induction of Tumor-specific Cytotoxic T Lymphocytes from Patients with Non-small Cell Lung Cancer Using CD8O-transfected Autologous Tumor Cells
• Interleukin-12 enhances the antitumor activity of cytotoxic T lymphocytes immunodeficient mice
• Indications and Operative Techniques for Combined Aortoesophageal Resection
• Effects of Interleukin-12 on the Induction of Cytotoxic T Lymphocytes from the Regional Lymph Node Lymphocytes of Patients with Lung Adenocarcinoma
• The Induction of Cytotoxic T Lymphocytes against HLA-A Locus-matched Lung Adenocarcinoma in Patients with Non-small Cell Lung Cancer
• Further Study on the Two Pivotal Parts of Hlg2 for the Full Hemolytic Activity of Staphylococcal γ-Hemolysin
• Sequential Binding of Staphylococcal γ-Hemolysin to Human Erythrocytes and Complex Formation of the Hemolysin on the Cell Surface
• Construction of a LukS-PV Mutant of a Staphylococcal Panton-Valentine Leukocidin Component having a High LukS-like Function
• An N-terminal Region of LukF of Staphylococcal Leukocidin/γ-Hemolysin Crucial for the Biological Activity of the Toxin
• Panton-Valentine Leukocidin Genes in a Phage-like Particle Isolated from Mitomycin C-Treated Staphylococcus aureus V8 (ATCC 49775)
• Efficient Production of Recombinant Human Erythropoietin by Replenishment of Microcarriers in the Hollow Fiber Culture Cassette
• Production of Recombinant Human Monoclonal Antibody Using ras-Amplified BHK-21 Cells in a Protein-free Medium
• イソマルトオリゴ糖摂取が健常人の便通と腸内環境に及ぼす影響
• Molecular Properties and Activity of a Carboxyl-Terminal Truncated Form of Xylanase 3 from Aeromonas caviae W-61
• Purification and Some Properties of High-molecular-weight Xylanases, the Xylanases 4 and 5 of Aeromonas caviae W-61
• Novel Characteristics of Selenomonas ruminantium Lysine Decarboxylase Capable of Decarboxylating Both L-Lysine and L-Ornithine
• Deactivation of Gibberellin by 2-Oxidation during Germination of Photoblastic Lettuce Seeds(Organic Chemistry)
• Cloning of the Maltose Phosphorylase Gene from Bacillus sp. Strain RK-1 and Efficient Production of the Cloned Gene and the Trehalose Phosphorylase Gene from Bacillus stearothermophilus SK-1 in Bacillus subtilis(Biochemistry & Molecular Biology)
• Characterization of Trehalose Phosphorylase from Bacillus stearothermophilus SK-1 and Nucleotide Sequence of the Corresponding Gene(Biochemistry & Molecular Biology)
• Purification and Characterization of Maltose Phosphorylase from Bacillus sp. RK-1(Biochemistry & Molecular Biology)
• REGULATION BY LIGHT AND LOCALIZATION OF GENE EXPRESSION OF GIBBERELLIN BIOSYNTHETIC ENZYMES DURING GERMINATION OF PHOTOBLASTIC LETTUCE SEEDS
• Regulation by Phytochrome of Gene Expression of Gibberellin Biosynthetic Enzymes during Germination of Photoblastic Lettuce Seeds
• LOCALIZATION OF GENE EXPRESSION OF GIBBERELLIN BIOSTNTHETIC ENZYME IN PHOTOBLASTIC LETTUCE SEEDS
• CLONING AND CHARACTERIZATION cDNAs ENCODING GIBBERELLIN BIOSYNTHETIC ENZYMES FROM LETTUCE
• Production of a Recombinant Human Monoclonal Antibody Using a Novel Hollow Fiber Bioreactor System
• Congenital tracheoesophageal fistula in an elderly patient with thoracic empyema
• Surgical treatment for chest wall invasion due to the local recurrence of breast cancer
• Lack and restoration of sensitivity of lung cancer cells to cellular attack with special reference to expression of human leukocyte antigen class I and/or major histocompatibility complex class I chain related molecules A/B
• Identification of tumor associated antigens recognized by IgG from tumor-infiltrating B cells of lung cancer : Correlation between Ab titer of the patient's sera and the clinical course
• Angiocentric Immunoproliferative Lesion of the Lung
• Analysis of MAGE-3 derived synthetic peptide as a human lung cancer antigen recognized by cytotoxic T lymphocytes
• Adoptive Immunotherapy Using Specific Cytotoxic T Lymphocytes Against Human Lung-Cancer-Engrafted Severe Combined Immunodeficiency Mice
• Induction of Tumor-Specific Cytotoxic T Lymphocytes from Regional Lymph Node Lymphocytes of Human Breast Cancer
• Autologous Tumor-specific Cytotoxic T Lymphocytes in a Patient with Lung Adenocarcinoma : Implications of the Shared Antigens Expressed in HLA-A24 Lung Cancer Cells
• Synthesis of Glycosyl Glycerol by Cyclodextrin Glucanotransferases(ENZYMOLOGY,PROTEIN ENGINEERING,AND ENZYME TECHNOLOGY)
• Purification and Some Properties of a β-Glucosidase from Flavobacterium johnsonae
• Purification and Characterization of a Novel β-Glucosidase from Clavibacter michiganense That Hydrolyzes Glucosyl Ester Linkage in Steviol Glycosides
• Photosystem II Inhibition by s-Triazines Having Hydrophilic Amino Groups
• A Simple Purification Method and Morphology and Component Analyses for Carotovoricin Er, a Phage-tail-like Bacteriocin from the Plant Pathogen Erwinia carotovora Er
• Assembly of Staphylococcus aureus Leukocidin into a Pore-Forming Ring-Shaped Oligomer on Human Polymorphonuclear Leukocytes and Rabbit Erythrocytes
• Digestibility of the Hydrogenated Derivative of an Isomaltooligosaccharide Mixture by Rats
• Action of Cyclodextrin Producing Enzyme(CGTase)and Diglucosyl-Cyclodextrins〔含 質疑応答〕 (1994年度糖質関連酵素化学シンポジウム)
• Identification of ribosomal protein L19 as a novel tumor antigen recognized by autologous cytotoxic T lymphocytes in lung adenocarcinoma
• Malignant mesothelioma-associated antigens recognized by tumor-infiltrating B cells and the clinical significance of the antibody titers
• SELECTIVE AND SPECIFIC DEGRADATION OF THED1 PROTEIN INDUCED BY BINDING OF A NOVEL PHOTOSYSTEM II INHIBITORTO THE Q_B -SITE
• Application of Mucin Histochemistry for Pathological Diagnosis : Expression of Gastric Phenotypes in Metaplastic and Neoplastic Lesions and its Relation to the Organoid Differentiation
• Histo- and Cytochemistry of Gland Mucus Cell Type Mucin and Its Practical Application for Pathologic and Clinical Diagnosis
• Histochemical Study of the Surface Mucous Gel Layer of Human Stomach.
• Ultrastructural Demonstration of Anionic Sites in the Basement Membrane of the Human Alveolar Type I Pneumocyte
• Mediastinal Metastasis from a Fibrolamellar Hepatocellular Carcinoma : Report of a Case
• EFFECT OF LIGHT ILLUMINATION ON THE SELECTIVE AND SPECIFIC DEGRADATION OF THE D1 PROTEIN INDUCED BY PHOTOSYSTEM II INHIBITOR PNO8
• Identification of a lung cancer antigen evading CTL attack due to loss of human leukocyte antigen (HLA) class I expression
• Evaluation of Acidogenicity of Commercial Isomaltooligosaccharides Mixture and Its Hydrogenated Derivative by Measurement of pH Response under Human Dental Plaque
• Identification of the Amino Acid Residues Conferring Substrate Specificity upon Selenomonas ruminantium Lysine Decarboxylase
• Identification of the Essential Amino Acid Residues in LukS for the Hemolytic Activity of Staphylococcal Leukocidin towards Rabbit Erythrocytes
• Differences in sensitivity to tumor-specific CTLs between primary and metastatic esophageal cancer cell lines derived from the same patient

もっと見る 閉じる

スポンサーリンク