Production of Recombinant Human Monoclonal Antibody Using ras-Amplified BHK-21 Cells in a Protein-free Medium
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概要
- 論文の詳細を見る
A ras oncogene-amplified recombinant BHK-21 cell line (ras-rBHK-IgG) has been established, and was shown to hyperproduce the recombinant IgG chimeric human monoclonal antibody (hMAb) AE6F4, which recognizes lung cancer cells. We found that the ras-rBHK-IgG cells could be easily cultured in a protein-free ERDF medium supplemented with iron(III) nitrate, hydroxyethyliminodiacetic acid, and non-protein synthetic attachment factor as well as in a serum-free ERDF medium supplemented with insulin, transferrin, ethanolamine, and sodium selenite. The productivity of recombinant hMAb from the cells cultured in dishes at high cell densities was higher in protein-free medium than in serum-containing medium. True high density culture of the ras-rBHK-IgG cells was done in protein-free medium using the Tecnomouse, which is a novel hollow fiber bioreactor system. After culture for 30 days in protein-free culture, a total amount of about 14 mg of the recombinant hMAb AE6F4 was obtained, and was shown to be reactive against lung cancer cells in tissues.
- 社団法人日本農芸化学会の論文
- 1996-05-23
著者
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NOMOTO Kikuo
Department of Immunology Kyushu University
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Inoue Yuichi
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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Kawamoto Seiji
Graduate School Of Genetic Resources Technology Kyushu University
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Kawamoto Seiji
Graduate School Of Advanced Sciences Of Matter Hiroshima University
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Tachibana H
Laboratory Of Food Chemistry Division Of Applied Biological Chemistry Department Of Bioscience And B
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Tachibana Hirofumi
九州大学 農学部生物機能化学食糧化学
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Tachibana Hirofumi
Graduate School Of Genetic Resources Technology Kyushu University
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Yamada Koji
Laboratory Of Food Chemistry Division Of Applied Biological Chemistry Department Of Bioscience And B
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Yazawa K
九州大学 農学部生物機能化学食糧化学
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Nomoto Kikuo
Department Of Immunology Medical Institute Of Bioregulation Kyasha University
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SEKI Kiyohiko
Department of Applied Biochemistry and Food Science, Faculty of Agriculture, Saga University
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Yamazaki Koji
産業医科大学 2外
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YASUMOTO Kosei
Second Department of Surgery, School of Medicine, University of Occupational and Environmental of He
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SHIRAHATA Sanetaka
Graduate School of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
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NAGASHIMA Akira
Department of Chest Surgery, Kitakyushu Municipal Medical Center
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TAKENOYAMA Mitsuhiro
Department of Surtzerv II, University of Occupational and Environmental Health
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OHASHI Hideya
Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd.
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Katakura Y
Fac. Of Agriculture Kyushu Univ.
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TERUYA Kiichiro
Graduate School of Systems Life Sciences, Kyushu University
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KATAKURA Yoshinori
Graduate School of Systems Life Sciences, Kyushu University
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ARITA Nobuhisa
Graduate School of Genetic Resources Technology, Kyushu University
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INOUE Yuichi
Graduate School of Genetic Resources Technology, Kyushu University
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LOPEZ Lourdes
Center of Molecular Immunology
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SHOJI Masahiro
Morinaga Institute of Biological Science
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KAMEI Masanori
Morinaga Institute of Biological Science
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HASHIZUME Shuichi
Morinaga Institute of Biological Science
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SHIOZAWA Yasumitsu
GUNZE SANGYO, INC.
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NAKAHASHI Hisashi
Matsuyama Red Cross Hospital
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SUZUKI Tatsuo
Department of Research Planning, Kitasato Institute Hospital
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IMAI Tadaaki
Section of Pathology, the Kitasato Institute Medical Center Hospital
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Inoue Y
Showa Sangyo Co. Ltd.
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Inoue Y
Solar Energy Research Group Institute For Physical And Chemical Research
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Inoue Yorinao
Photosynthesis Research Lab.
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OHASHI Hideo
Faculty of Agriculture, Gifu University
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Tachibana Hirofumi
Department Of Food Science And Technology Faculty Of Agriculture Kyushu University
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Yasumoto K
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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Yamada K
Laboratory Of Food Chemistry Division Of Applied Biological Chemistry Department Of Bioscience And B
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Yamada Koji
Department Of Food Science And Technology And Graduate School Of Genetic Resources Technology Facult
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So Tetsuya
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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Seki K
Chiba Univ. Chiba Jpn
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Seki Kiyohiko
Department Of Applied Biological Sciences Faculty Of Agriculture Saga University
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Ohashi H
Kirin Brewery Co. Ltd. Gunma Jpn
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Imai Tadaaki
Section Of Pathology The Kitasato Institute Medical Center Hospital
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Ohashi Hideya
Pharmaceutical Research Laboratory Kirin Brewery Co. Ltd.
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Seki Kiyohiko
Department Of Applied Biochemistry And Food Science Faculty Of Agriculture Saga University
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Arita Nobuhisa
Graduate School Of Genetic Resources Technology Kyushu University
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Kamei Masanori
Morinaga & Co. Ltd.
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Teruya Kiichiro
Fac. Of Agriculture Kyushu Univ.
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Shirahata Sanetaka
Graduate School Of Systems Life Sciences Kyushu University:department Of Genetic Resources Technolog
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Shirahata S
Fac. Of Agriculture Kyushu Univ.
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Shirahata Sanetaka
Graduate School Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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Tachibana H
Faculty Of Agriculture Kyushu University
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Yasumoto Kosei
Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health
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Yamada K
Lab. Of Nutrition Chemistry Dep. Of Bioscience And Biotechnology Fac. Of Agriculture Graduate School
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Katakura Yoshinori
Dep. Of Genetic Resources Technol. Fac. Of Agriculture Kyushu Univ.
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Nagashima A
Department Of Chest Surgery Kitakyushu Municipal Medical Center
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Nakahashi Hisashi
Department Of Respiratory Surgery Matsuyama Red Cross Hospital
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Suzuki Tatsuo
Department Of Neuroplasticity Research Center On Aging And Adaptation Shinshu University School Of M
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Katakura Yoshinori
Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University
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Teruya Kiichiro
Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University
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Shiozawa Yasumitsu
Gunze Sangyo Inc.
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Tian Huaize
Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu University
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Suzuki Tatsuo
Department Of Neuroplasticity Institute On Aging And Adaptation Shinshu University Graduate School O
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Suzuki Tatsuo
Department Of Biology Faculty Of Science Osaka University
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Nagashima Akira
Department Of Chest Surgery Kitakyushu Municipal Medical Center
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Suzuki Tatsuo
Department Of Biochemistry Nagoya City University Medical School
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Yamada Koji
Laboratory Of Food Chemistry Division Of Applied Biological Chemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu University
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Yamada Kazuya
Department Of Nutrition And Physiological Chemistry Osaka University Medical School
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Nagashima Akira
Department Of Chest Surgery And Pathology Kitakyushu Municipal Medical Center
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Takenoyama Mitsuhiro
Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
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Nagashima Akira
Department of Chemical Engineering, Tokyo Institute of Technology
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SHIRAHATA Sanetaka
Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
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