Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-producing Cell Lines

概要

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To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been`primed' by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.
社団法人日本農芸化学会の論文
1995-02-23