Human-Human Hybridomas Secreting IgM-like Immunoglobulin with α and μ Heavy Chains(Biological Chemistry)
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概要
- 論文の詳細を見る
Human-human hybridomas were produced by fusion of a malignant 6-thioguanine resistant human B lymphoblastoid cell line and normal lymphocytes. The fusion efficiency with lymphocytes from peripheral blood was elevated by preculture of the lymphocytes in the presence of lipopolysaccharides. However, the preculture did not enhance the efficiency with lymphocytes from lung cancer tissue. The resulting hybridomas secreted mostly IgG and/or IgM. A small number of hybridomas secreted IgA. Two IgA secreting hybridomas, 9P-13-4 and 9L-27-9 also produced IgM-like immunoglobulin even after repeated cloning, indicating these hybridomas secrete IgA and IgM-like immunoglobulins simultaneously. The IgM-like immunoglobulin has a molecular weight of 900K and contains 3 monomeric IgMs and 2 IgAs. This indicates that it is therefore possible to produce a IgM-like immunoglobulin binding to two different antigen determinants, by hybridoma technology.
- 社団法人日本農芸化学会の論文
- 1986-09-23
著者
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SHINMOTO Hiroshi
Technical Research Institute, Snow Brand Milk Products Co., Ltd.
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Murakami H
Graduate School Of Genetic Resources Technology Kyushu University
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Murakami Hiroki
Laboratory Of Food Chemistry Faculty Of Agriculture Kyushu University
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Hirose S
Tokachi‐ikeda Research Inst. Viticulture And Enology Hokkaido Jpn
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Shinmoto H
Snow Brand Milk Products Co. Ltd. Kawagoe Jpn
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Omura Hirohisa
Institute Of Food Science And Technology Faculty Of Agriculture Kyushu University
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Omura Hirohisa
Department Of Food And Nutrition Nakamura-gakuen College
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Omura Hirohisa
Food Chemistry Institute Faculty Of Agriculture Kyushu University
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Omura Hirohisa
Laboratory Of Food Chemistry Faculty Of Agriculture Kyushu University
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MURAKAMI Hiroki
Institute of Food Science and Technology, Faculty of Agriculture, Kyushu University
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Shinohara Kazuki
Institute of Food Chemistry, Faculty of Agriculture, Kyushu University
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Omura H
Kyushu Univ. Fukuoka Jpn
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Shinohara K
National Food Research Institute
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Shinohara Kazuki
National Food Research Institute
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Shinohara Kazuki
Laboratory Of Food Chemistry Faculty Of Agriculture Kyushu University
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Dosako S
Snow Brand Co. Ltd. Saitama Jpn
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Dosako S
Technical Research Institute Snow Brand Milk Products Co. Ltd.
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Shinohara Kazuki
Food Chemistry Institute Faculty Of Agriculture Kyushu University
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Shinmoto Hiroshi
Technical Research Institute Snow Brand Milk Products Co. Ltd.
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