Synthesis of Bis-piperazine-type pH Buffer Agents and Their Application to Mammalian Cell Cultures
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概要
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New bis-piperazine-type pH buffer agents were synthesized and their buffering properties were evaluated. The compounds proved to have two-fold larger pH buffering ability than 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES), a Good's buffer traditionally used to control the pH value of culture media. Human-human hybridoma HB4C5 cells were cultured in a serum-free medium containing these buffer agents. The cell growth and antibody production, using 1, 2-N, N'-bis[N", N"'-di(2-sulfonoethyl)piper-azinyl]ethane, were greater than when HEPES.
- 社団法人日本農芸化学会の論文
- 1994-08-23
著者
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Murakami Hiromi
Osaka Municipal Technical Res. Inst.
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Nagira Kazuhiko
Dojindo Laboratories
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Sugahara T
Ehime Univ. Ehime Jpn
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Sugahara Takuya
Laboratory Of Cellular Regulation Technology Graduate School Of Genetic Resources Technology Kyushu
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Murakami Hiroki
Laboratory Of Food Chemistry Faculty Of Agriculture Kyushu University
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Murakami H
Laboratory Of Cellular Regulation Technology Graduate School Of Genetic Resources Technology Kyushu
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SHIGA Masanobu
Dojindo Laboratories
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SASAMOTO Kazumi
Dojindo Laboratories
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Shiga Masanobu
Dojindo Laboratores
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Sasamoto Kazumi
Dojindo Laboratores
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Murakami H
Osaka Municipal Technical Res. Inst.
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HAYASHIDA Midori
Laboratory of Cellular Regulation Technology, Graduate School of Genetic Resources Technology, Kyush
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Hayashida Midori
Laboratory Of Cellular Regulation Technology Graduate School Of Genetic Resources Technology Kyushu
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Kina Ken'yu
Dojindo Laboratories
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