Stimulation of IgM Production in Human-Human Hybridoma HB4C5 Cells by Chitosan
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概要
- 論文の詳細を見る
We screened for immunoglobulin production stimulating factors (IPSFs) in polysaccharides using human-human hybridoma cells, HB4C5,cultured in serum-free medium. Among polysaccharides, citrus pectin, locust bean gum, and chitosan stimulated IgM production of HB4C5 cells. Especially chitosan showed the strongest IPSF activity ; 100 ng/ml of chitosan stimulated IgM production approximately 5-fold. Chitosan had several characteristics as IPSF, as follows. 1) For the IPSF activity, 70-90% deacetylation was essential. 2) Chitosan oligomers (n=5,6,7) and chitin oligomers (n=5,6,7) showed no IPSF activities. 3) The IPSF activity of chitosan was inhibited by glucosamine, one of the constitutive sugars of chitosan. 4) Chitosan stimulated IgM production of human lymphocytes in serum-free culture, but not IgG or IgA, nor in serum-supplemented culture.
- 社団法人日本農芸化学会の論文
- 1992-03-23
著者
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Murakami Hiromi
Osaka Municipal Technical Res. Inst.
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Ohta Hideaki
Chugoku National Agricultural Experiment Station, Ministry of Agriculture, Forestry, and Fisheries
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Tajima Makoto
Faculty of Health Science, Kobe University School of Medicine
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Murakami Hiroki
Graduate School of Genetic Resources Technology, Kyushu University
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Murakami H
Kyushu Univ. Fukuoka Jpn
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Murakami H
Graduate School Of Genetic Resources Technology Kyushu University
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Murakami H
Laboratory Of Cellular Regulation Technology Graduate School Of Genetic Resources Technology Kyushu
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MAEDA Mari
Chugoku National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries
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Ohta Hideaki
Chugoku National Agricultural Experiment Station Ministry Of Agriculture Forestry And Fisheries
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Maeda Mari
Chugoku National Agricultural Experiment Station Ministry Of Agriculture Forestry And Fisheries
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Tajima Makoto
Faculty Of Home Economics Jissen Women's University
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Tajima Makoto
Faculty Of Health Science Kobe University School Of Medicine
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Murakami H
Osaka Municipal Technical Res. Inst.
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Murakami Hiroki
Graduate School Of Genetic Resources Technology Kyushu University
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Murakami Hiroki
Graduate School Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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