Enhancement of Immunoglobulin Productivity of Human-Human Hybridoma HB4C5Cells by Basic Proteins and Poly-basic Amino Acids
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概要
- 論文の詳細を見る
Immunoglobulin M productivity of a human-human hybridoma line, HB4C5, was enhanced by the addition of histones H1, H2A, and H2B in serum-free medium, which are lysine-rich histones. On the contrary arginine-rich histones, histone H3 and H4, did not stimulate immunoglobulin production at all. Another arginine-rich protein, protamine also did not significantly stimulate immunoglobulin production. These results suggested that there is a correlation between amino acid residues of basic proteins and stimulation of immunoglobulin production. In this connection, immunoglobulin production stimulating activities of poly-lysine and poly-arginine were investigated. Both poly-L-lysine and poly-D-lysine stimulated IgM productivity of the human-human hybridomas. These results show that the enhancing effect of lysine residues was derived from its physiochemical features. On the other hand, poly-L-arginine did not enhance the productivity.
- 社団法人日本農芸化学会の論文
- 1994-12-23
著者
-
Sasaki Takeshi
Department Of Biotechnology College Of Agriculture Ehime University
-
Sugahara Takuya
Department Of Biotechnology College Of Agriculture Ehime University
-
Murakami Hiroki
Graduate School Of Genetic Resources Technology Faculty Of Agriculture Kyushu University
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Sasaki T
Ehime Univ. Ehime Jpn
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Sasaki Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Ehime University
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