Synthesis of Novel Heterobranched β-Cyclodextrins from 4^2-O-β-D-Galactosyl-Maltose and β-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products

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概要

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• From the mixture of 4^2-O-β-D-galactosyl-maltose (Gal-G2) and β-cyclodextrin (βCD), novel heterobranched βCDs, (Gal-G2)-βCD and (Gal-G2)_2-βCDs, were synthesized by the reverse action of debranching enzyme. The optimum conditions for the production of (Gal-G2)_2-βCDs were examined. A mixture of (Gal-G2)_2-βCDs was produced in about 4% yield when Aerobacter aerogenes pullulanase (64 units per 1g of Gal-G2) was incubated with 1.6M Gal-G2 and 0.16M βCD at 50℃ for 4 days. The reaction products, (Gal-G2)_2-βCDs, were separated into three peaks by HPLC analysis on a Hypercarb S column. Their structures were analyzed by fast atom bombardment mass spectroscopy and NMR spectroscopies, and confirmed by comparison of their hydrolyzates by β-galactosidase with the authentic (G2)_2-βCDs. The structures of (Gal-G2)-βCD and three components of (Gal-G2)_2-βCDs were identified as 6-O-(Gal-G2)-βCD, 6^1,6^2-, 6^1,6^3- and 6^1,6^4-di-O-(Gal-G2)_2-βCD, respectively.

• 社団法人日本農芸化学会の論文
• 2000-06-23

著者

• Murakami Hiromi

  Osaka Municipal Technical Res. Inst.

• Hashimoto H

  Bio Research Corporation Of Yokohama

• Hashimoto Hitoshi

  Materials Research Institute For Sustainable Development National Institute Of Advanced Industrial S

• TANIMOTO Toshiko

  School of Pharmaceutical Sciences Mukogawa Women's University

• Fujita K

  Kyoto Prefectural Univ. Kyoto Jpn

• Fujita Koki

  Bio Research Corporation Of Yokohama

• Tanaka K

  Tottori Univ. Tottori

• KOIZUMI Kyoko

  School of Pharmaceutical Sciences, Mukogawa Women's University

• HASHIMOTO Hitoshi

  Bio Research Corporation of Yokohama

• Koizumi K

  School Of Pharmaceutical Sciences Mukogawa Women's University

• Koizumi Kyoko

  School Of Pharmaceutical Sciences Mukogawa Women's University

• Hashimoto H

  Materials Research Institute For Sustainable Development National Institute Of Advanced Industrial S

• Hashimoto Hitoshi

  Res. Inst. For Sustainable Materials National Inst. Of Advanced Industrial Sci. And Technol.

• KITAHATA Sumio

  Osaka Municipal Technical Research Institute

• Murakami H

  Laboratory Of Cellular Regulation Technology Graduate School Of Genetic Resources Technology Kyushu

• NAKANO HIROFUMI

  Osaka Municipal Technical Research Institute, and Yashiro Co, Ltd.,

• Tanaka Kiyoshi

  Coll. Environ. Health Azabu Univ.

• Kitahata S

  Department Of Bioscience And Biotecnology Shinshu University

• IKUTA Akiko

  School of Pharmaceutical Sciences, Mukogawa Women's University

• TANAKA Keiko

  School of Pharmaceutical Sciences, Mukogawa Women's University

• Ikuta Akiko

  School Of Pharmaceutical Sciences Mukogawa Women's University

• Murakami H

  Osaka Municipal Technical Res. Inst.

• Kobayashi S

  Fukui Univ. Technol. Fukui Jpn

• Nakano H

  Department Of Biochemistry Osaka Municipal Technical Research Institute

• Furuichi K

  Bio Research Corporation Of Yokohama

• Tanimoto T

  School Of Pharmaceutical Sciences Mukogawa Women's University

• Tanimoto Toshiko

  School Of Pharmaceutical Science Mukogawa Women's University

• Nakano H

  Kyoto Prefectural Univ. Kyoto Jpn

• Nakano Hirofumi

  Osaka Municipal Technical Research Institute

• Kikuchi Takao

  Coll. Environ. Health Azabu Univ.

• Hashimoto H

  Department Of Biotechnology The University Of Tokyo

• Nakano Hirofumi

  Department Of Chemistry Faculty Of Science Osaka University

• Tanaka Kyoko

  Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

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