Purification and Some Properties of β-Fructofuranosidase I from Arthrobacter sp. K-1(Biological Chemistry)

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概要

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• Anhrobacter sp. K-1, isolated from soil, produces β-fructofuranosidase. This enzyme preparation was separated into three fractions (I, II, and III) by butyl-Toyopearl 650 M column chromatography. The β-fructofuranosidase I was purified to homogeneity by disc-electrophoresis after consecutive column chromatographies. The enzyme had a molecular weight of 52,000 by SDS-polyacrylamide gel electrophoresis and 51,000 by gel filtration with Ultrogel AcA 44, and an isoelectric point of 4.3. The enzyme was most active at pH 6.5-6.8 and at 55℃ and stable up to 45℃ at pH 6.5 for 30 min of incubation, and from pH 5.5 to 10.0 at 40℃ in 2 hr of incubation. This enzyme hydrolyzed sucrose, erlose, raffinose, fructosylxyloside, neokestose, and stachyose at the relative velocities of 100, 75, 61, 59, 54, and 11, but hardly hydrolyzed 1-kestose and nystose (<0.02).

• 社団法人日本農芸化学会の論文
• 1990-04-23

著者

• Hashimoto H

  Bio Research Corporation Of Yokohama

• Hashimoto Hitoshi

  Materials Research Institute For Sustainable Development National Institute Of Advanced Industrial S

• HARA Kozo

  Bio Research Corporation of Yokohama

• Hara Koji

  Bio Research Corporation Of Yokohama

• Fujita K

  Kyoto Prefectural Univ. Kyoto Jpn

• Hara Kozo

  Carbohydrate Research Laboratories Ensuiko Sugar Refining Co. Ltd.

• Hashimoto H

  Materials Research Institute For Sustainable Development National Institute Of Advanced Industrial S

• Hashimoto Hitoshi

  Res. Inst. For Sustainable Materials National Inst. Of Advanced Industrial Sci. And Technol.

• KITAHATA Sumio

  Osaka Municipal Technical Research Institute

• FUJITA Koki

  Carbohydrate Research Laboratory, Ensuiko Sugar Refining Co., Ltd.

• HASHIMOTO Hitoshi

  Carbohydrate Research Laboratory

• Kitahata S

  Department Of Bioscience And Biotecnology Shinshu University

• Furuichi K

  Bio Research Corporation Of Yokohama

• Fujita Koki

  Carbohydrate Research Laboratory Ensuiko Sugar Refining Co. Ltd.

• Hashimoto H

  Department Of Biotechnology The University Of Tokyo

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