Continuous Production of Glucosyl-cyclodextrins Using Immobilized Cyclomaltodextrin Glucanotransferase(Microbiology & Fermentation Industry)

概要

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Immobilization by covalent bonding of cyclomaltodextrin glucanotransferase (CCTase) of Bacillus stearothermophilus was studied for the continuous production of glucosyl-cyclodextrins (G_1-CDs) from branched dextrins. A macroreticular hydrophilic resin containing a primary amine as the active group was synthesized as a candidate carrier of CGTase. The properties of CGTase immobilized on this carrier resin were examined and compared with those of the native enzyme. The optimum pH of the native enzyme is 6.0, but that of the immobilized enzyme extends from 6.0 to 8.0. The optimum temperature for the immobilized enzyme shifts slightly to a lower range than that of the native enzyme. In a column test there was an optimum range of substrate flow rate that maximized the G_1-CDs content in the column effluent. The G_1-CD content in the column effluent was greatly influenced by the substrate concentration, but not by the activity of the immobilized enzyme. We produced G_1-CDs continuously using a pilot-scale CGTase bioreactor. We could do the continuous operation to obtain an average G_1-CDs content of 10% (w/w) in the reactor effluent for 70 days.
社団法人日本農芸化学会の論文
1991-01-23