Galactosylation of Thiol Group by β-Galactosidase

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概要

• 論文の詳細を見る

• β-Galactosidase catalyzed β-galactosylation not only of a hydroxyl group but also of a thiol group in the condensation reaction of D-galactose and 2-mercaptoethanol. The thio-galactosylation product was confirmed as 2-hydroxyethyl S-β-D-galactoside on the bases of fast atom bombardment mass spectrometry, infrared spectroscopy, and nuclear magnetic resonance spectorometry. Aspergillus oryzae β-galactosidase hydrolyzed p-nitrophenyl S-β-D-galactoside most rapidly among several β-galactosidases and produced the thio-galactosylation product most efficiently. The Penicillim multicolor enzyme was as effective as the A. oryzae enzyme. However the enzymes from Escherichia coli, Saccharomyces fragilis, Kluyveromyces lactis, and Bacillus circulans galactosylated hydroxyl groups predominantly to produce 0-galactoside. The thio-galactoside was synthesized most effectively at a 2-mercaptoethanol concentration of about 1.25M. Galactose concentration at 0.8-2.8M did not affect the synthetic yield of the thio-galactoside so greatly.

• 社団法人日本農芸化学会の論文
• 2000-04-23

著者

• Shizuma Motohiro

  Osaka Municipal Technical Research Institute

• KITAHATA Sumio

  Osaka Municipal Technical Research Institute

• KISO TARO

  Osaka Municipal Technical Research Institute

• NAKANO HIROFUMI

  Osaka Municipal Technical Research Institute, and Yashiro Co, Ltd.,

• Shizuma Motohiro

  Technochemistry Osaka Municipal Technical Research Institute

• Kitahata S

  Department Of Bioscience And Biotecnology Shinshu University

• SHIZUMA Motohiro

  Department of Biochemistry, Osaka Municipal Technical Research Institute

• Kiso Taro

  Department Of Biochemistry Osaka Municipal Technical Research Institute

• Kobayashi S

  Fukui Univ. Technol. Fukui Jpn

• Shizuma Motohiro

  Department Of Biochemistry Osaka Municipal Technical Research Institute

• Nakano H

  Department Of Biochemistry Osaka Municipal Technical Research Institute

• Nakano H

  Kyoto Prefectural Univ. Kyoto Jpn

• Nakano Hirofumi

  Osaka Municipal Technical Research Institute

• Nakano Hirofumi

  Department Of Chemistry Faculty Of Science Osaka University

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