Fatty Acid Specificity of Rhizopus delemar Lipase in Acidolysis
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概要
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We investigated the fatty acid specificity of 1,3-positional specific Rhizopus delemar lipase in acidolysis using a randomly interesterified oil, of which the consituent fatty acid contents were approximately the same, as a substrate. Acidolysis was carried out at 30℃ with shaking in a eraction mixture containing caprylic acid (CA)/the randomlyinteresterified oil (2 : 1,w/w) and the lipase immpbilized on a ceramic carrier (4% of reaction mixture). The activity on each fatty acid was experimentally determined as the ratio of the content of the fatty acid exchanged for CA to that of the fatty acid existing at the 1(3)-position of the substrate oil, and regarded as the fatty acid specificity in acidolysis. The fatty acid specificity in hydrolysis was in vestigated by analysis of the composition of fatty acids liberated from the same randomly interesterified oil. The lipase acted strongly on myristic, palmitic, palmitoleic, stearic, oleic, linoleic, and α-linolenic acids, and moderately on arachidonic and eicosapentaenoic acids. The activities on these fatty acids relative to that on oleic acid were almost the same in acidolysis and hydrolysis. On the other hand, the lipase acted moderately on γ-linolenic and docosahexaenoic acids in the hydrolysis, but only very weakly on these fatty acids in the early stage of acidolysis. This shows that the fatty acid specificity in acidolysis is stricter than that in hydrolysis. Since the kinetics of acidolysis of borage oil, arachidonic acid-containing oil (TGA-25), and tuna oil could be easily predicted from the fatty acid specificity in acidolysis, it was concluded that the specificity was accurately evaluated. In addition, the fatty acids at the 1(3)-position of borage oil and TGA-25 were completely exchanged for CA in a total of three cycles of the reaction, and the contents of fatty acids other than CA in the transesterified oils agreed with that at the 2-position of the oils. Therefore, it was shown that repeated acidolysis using the immobilized 1,3-soecufuc lipase could be applied to reglospecific analysis.
- 社団法人日本生物工学会の論文
- 1997-04-25
著者
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Tominaga Y
Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Yanagita Teruyoshi
Lab. Of Nutrition Biochemistry Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Nakai Seiichi
Osaka Municipal Technical Research Institute And Yashiro Co Ltd.
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Shimada Y
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Shimada Y
Department Of Physics Chuo University:tecmo
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Shimada Yuji
Osaka Municipal Technical Research Institute
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NAGAO Toshihiro
Osaka Municipal Technical Research Institute
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SUGIHARA Akio
Faculty of Engineering, Tokushima Bunri University
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SUGIHARA Akio
Osaka Municipal Technical Research Institute
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TOMINAGA Yoshio
Osaka Municipal Technical Research Institute
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NAKANO HIROFUMI
Osaka Municipal Technical Research Institute, and Yashiro Co, Ltd.,
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SUENAGA MASAHARU
Osaka Municipal Technical Research Institute, and Yashiro Co, Ltd.,
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Yanagita Teruyoshi
Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Tominaga Yoshio
The Organ Transplant Center Nagoya Second Red Cross Hospital
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Sugihara A
Faculty Of Engineering Tokushima Bunri University
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Nagao Toshihiro
Biomaterials And Commodity Chemicals Res. Div. Osaka Municipal Technical Res. Inst.
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Nagao Toshihiro
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Nakano Hirofumi
Osaka Municipal Technical Research Institute
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Suenaga Masaharu
Osaka Municipal Technical Research Institute And Yashiro Co Ltd.
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Nakano Hirofumi
Department Of Chemistry Faculty Of Science Osaka University
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