Fractionation of Conjugated Linoleic Acid Isomers by Selective Hydrolosis with Candida rugosa Lipase
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概要
- 論文の詳細を見る
It was attempted to prepare cis-9, trans-11 conjugated linoleic acid (c9,t11-CLA) and t10,c12-CLA concentrates that can be used as foods. A free fatty acid mixture (FFA-CLA) containing almost equal amounts of c9,t11- and t10,c12-CLAs was esterified with glycerol using immobilized Rhizomucor miehei lipase, and the resulting acylglycerols (Gly-CLA) were purified by molecular distillation. Contents of c9,t11- and t10,c12-CLAs in Gly-CLA were the same as those in FFA-CLA: c9,t11-CLA, 33.7 wt%; t10,c12-CLA, 34.5 wt%. Gly-CLA was first hydrolyzed with an equal weight of water and 1.0 U/g-mixture of Candida rugosa lipase, and c9,t11-CLA-rich FFAs were prepared by molecular distillation: purity of c9,t11-CLA based on the total content of c9,t11- and t10,c12-CLAs, 72.9%. Meanwhile, purity of t10,c12-CLA in acylglycerols was 65.0%. To further increase the purity, the acylglycerols were hydrolyzed again with 15 U/g-mixture of C. rugosa lipase, resulting in enrichment of t10,c12-CLA in acylglycerols (purity of t10,c12-CLA, 80.4%). Non-selective hydrolysis of t10,c12-CLA-rich acylglycerols with 200 U/g-mixture of C. rugosa lipase produced t10,c12-CLA-rich FFAs (purity of t10,c12-CLA, 81.5%). In addition, c9,t11-CLA-rich FFAs were successfully esterified with glycerol using immobilized R. miehei lipase, and c9,t11-CLA-rich acylglycerols can be synthesized (purity of c9,t11-CLA, 73.0%). The process was composed of reactions with C. rugosa and R. miehei lipases, which can be used for production of foods, and molecular distillation. Hence, the c9,t11- and t10,c12-CLA concentrates can be used as foods.
- 日本油化学会の論文
- 2003-07-01
著者
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Shimada Y
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Shimada Y
Department Of Physics Chuo University:tecmo
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Shimada Yuji
Osaka Municipal Technical Research Institute
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TERAI Tadamasa
Department of Applied Chemistry, Osaka Institute of Technology
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Terai Tadamasa
Dep. Of Applied Chemistry Osaka Inst. Of Technol.
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NAGAO Toshihiro
Osaka Municipal Technical Research Institute
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YAMAUCHI-SATO Yoshie
Rinoru Oil Mills Co., Ltd.
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SUGIHARA Akio
Faculty of Engineering, Tokushima Bunri University
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Yamamoto Takaya
Rinoru Oil Mills Co. Ltd.
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Yamauchi-sato Yoshie
Res. Lab. The Nisshin Oillio Group Ltd.
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Sugihara A
Faculty Of Engineering Tokushima Bunri University
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Terai Tadamasa
Department Of Applied Chemistry Osaka Institute Of Technology
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Yamamoto T
Aist Tsukuba Jpn
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Nagao Toshihiro
Biomaterials And Commodity Chemicals Res. Div. Osaka Municipal Technical Res. Inst.
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Nagao Toshihiro
Department Of Biochemistry Osaka Municipal Technical Research Institute
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SUGIHARA Akio
Environmental and Systems Engineering, Faculty of Engineering, Tokushima Bunri University
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Terai T
Dep. Of Applied Chemistry Osaka Inst. Of Technol.
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YAMAUCHI-SATO Yoshie
Rinoru Oil Mills Co. Ltd.
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