Cloning, Nucleotide Sequencing, and Expression in Escherichia coli of a Lipase and Its Activator Genes from Pseudomonas sp. KWI-56(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
A lipase gene (lip) and its activator gene (act) on a 2.9kb BglII-EcoRI fragment from Pseudomonas sp. KWI-56 were cloned in Escherichia coli using pUC19 as a vector plasmid. From the sequencing results, the open reading frames of the lip and the act were found to contain 1092 and 1032 nucleotides, respectively. The act existed downstream of the lip with the same orientation. When the lip was expressed in E. coli using the lac promoter on the pUC plasmid vector, the lipase activity of E. coli carrying both the lip and the act was 200-fold greater than that carrying only the lip. This result suggested the act was important in the expression of the lip in E. coli.
- 社団法人日本農芸化学会の論文
- 1991-09-23
著者
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Tominaga Y
Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Yanagita Teruyoshi
Lab. Of Nutrition Biochemistry Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Shimada Y
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Shimada Y
Department Of Physics Chuo University:tecmo
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Shimada Yuji
Osaka Municipal Technical Research Institute
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Nagao Koji
Department Of Applied Biological Sciences Saga University
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SUGIHARA Akio
Osaka Municipal Technical Research Institute
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TOMINAGA Yoshio
Osaka Municipal Technical Research Institute
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Yanagita Teruyoshi
Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Tominaga Yoshio
The Organ Transplant Center Nagoya Second Red Cross Hospital
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Sugihara A
Faculty Of Engineering Tokushima Bunri University
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Nakamura K
National Institute Of Bioscience And Human Technology
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Iizumi Taro
Kurita Central Laboratories, Kurita Water Industries Ltd.
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Nakamura Koichi
Kurita Central Laboratories, Kurita Water Industries Ltd.
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Fukase Tetsuro
Kurita Central Laboratories, Kurita Water Industries Ltd.
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Fukase T
Kurita Water Ind. Ltd. Atsugi‐shi Jpn
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Fukase Tetsuro
Kurita Central Laboratories Kurita Water Industries Ltd.
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Iizumi T
Kurita Water Ind. Ltd. Atsugi‐city Jpn
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IZUMI Taro
Kurita Central Laboratories, Kurita Water Industries Ltd.
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Nakamura Koichi
Kurita Central Laboratories Kurita Water Industries Ltd
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NAKAMURA KAZUNORI
Kurita Central Laboratories, Kurita Water Industries Ltd.
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