Purification and Characterization of a Carboxylesterase from Pseudomonas sp. KWI-56
スポンサーリンク
概要
- 論文の詳細を見る
An intracellular carboxylesterase from Pseudomonas sp. was overproduced in E. coli, and purified to homogeneity by a combination of hydrogen bond chromatography, gel filtration, and hydrophobic interaction chromatography. Gel filtration and SDS-PAGE suggested that the purified enzyme consisted of two subunits of molecular mass of 28kDa. Its isoelectric point was 5.9. The enzyme was thermolabile, and showed its maximum activity at 22℃ (pH 7.5). Methyl propionate was hydrolyzed at the highest rate among the fatty acid methyl esters tested. PMSF, DFP, PCMB, and HgCl_2 inhibited the enzyme markedly, suggesting that serine and/or cysteine is in or near the active site.
- 社団法人日本農芸化学会の論文
- 1994-04-23
著者
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Tominaga Y
Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Yanagita Teruyoshi
Lab. Of Nutrition Biochemistry Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Shimada Y
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Shimada Y
Department Of Physics Chuo University:tecmo
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Shimada Yuji
Osaka Municipal Technical Research Institute
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Nagao Koji
Department Of Applied Biological Sciences Saga University
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NAGAO Toshihiro
Osaka Municipal Technical Research Institute
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SUGIHARA Akio
Faculty of Engineering, Tokushima Bunri University
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SUGIHARA Akio
Osaka Municipal Technical Research Institute
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TOMINAGA Yoshio
Osaka Municipal Technical Research Institute
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Yanagita Teruyoshi
Dep. Of Applied Biochemistry And Food Sci. Saga Univ.
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Tominaga Yoshio
The Organ Transplant Center Nagoya Second Red Cross Hospital
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Sugihara A
Faculty Of Engineering Tokushima Bunri University
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Nagao Toshihiro
Biomaterials And Commodity Chemicals Res. Div. Osaka Municipal Technical Res. Inst.
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Nagao Toshihiro
Department Of Biochemistry Osaka Municipal Technical Research Institute
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Nakamura K
National Institute Of Bioscience And Human Technology
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Iizumi Taro
Kurita Central Laboratories, Kurita Water Industries Ltd.
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Nakamura Koichi
Kurita Central Laboratories, Kurita Water Industries Ltd.
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Fukase Tetsuro
Kurita Central Laboratories, Kurita Water Industries Ltd.
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Fukase T
Kurita Water Ind. Ltd. Atsugi‐shi Jpn
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Fukase Tetsuro
Kurita Central Laboratories Kurita Water Industries Ltd.
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Iizumi T
Kurita Water Ind. Ltd. Atsugi‐city Jpn
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Iizumi Taro
Kurita Central Laboratories Kurita Water Industries Ltd.
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Nakamura Koichi
Kurita Central Laboratories Kurita Water Industries Ltd
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NAKAMURA KAZUNORI
Kurita Central Laboratories, Kurita Water Industries Ltd.
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