Isolation, Characterization and Identification of Polyhydroxyalkanoate-Accumulating Bacteria from Activated Sludge

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概要

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• Two novel gram-positive bacteria capable of accumulation poly(3-hydroxybutyrate-co-3-hydroxyvalerate)[poly(3HB-co-3HV)] were isolated from an anaerobic-oxic activated sludge system fed with acetate. Strains Lpha5 and Lpha7 are motile cocci, 1-2 μm in diameter, occurring singly or in pairs. These isolates have doubling times ranging from 0.4-1.7 d and can accumulate in high levels of poly(3HB-co-3HV)(up to 44.7% of cell dry weight) when grown on complex media. Furthermore, these two strains exhibited the rapid substrate uptake and accumulation of storage granules as observed in situ. Under aerobic conditions, about 14.4%(cell dry weight)polyhydroxyalkanoate and 82%(carbon dry weight) cellular carbohydrate were produced from acetate and glucose, respectively. Under anaerobic conditions, poly(3HB-co-3HV) and cellular carbohydrate accumulated when glucose but not acetate was fed. The result of analysis of 16S rRNA sequence revealed that both straing belons to the gram-positive high-G+C group, but are significantly different from their closest phylogenetic relatives, Dermatophilus sp.and Terrabacter sp., to warrant classification as a new species.

• 社団法人日本生物工学会の論文
• 2000-11-25

著者

• Mino Takashi

  Graduate School Of Engineering Chiba Univ.

• Mino Takashi

  Department Of Urban And Environmental Engineering University Of Tokyo

• Liu W‐t

  National Central Univ. Chungli Twn

• Liu Wen-tso

  Division Of Environmental Science And Engineering National University Of Singapore

• LIU WEN-TSO

  Graduate Institute of Environmental Engineering, National Central University

• Nagao Koji

  Department Of Applied Biological Sciences Saga University

• NAKAMURA KAZUNORI

  National Institute of Advanced Industrial Science and Technology (AIST)

• Liu Wen-tso

  Graduate Institute Of Environmental Engineering National Central University

• Nakamura K

  National Institute Of Bioscience And Human Technology

• MATSUO TOMONORI

  Department of Urban and Environmental Engineering, University of Tokyo

• Matsuo T

  Department Of Urban And Environmental Engineering Universitt Of Tokyo

• MINO TAKASHI

  Department of Materials Technology, Faculty of Engineering, Chiba University

• MINO TAKASHI

  Department of Urban and Environmental Engineering, University of Tokyo

• Mino Takashi

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Chiba University

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