Quantification of Rhodocyclus-Related and Actinobacterial Polyphosphate-Accumulating Organisms in an Enhanced Biological Phosphorus Removal Process Using Quenching Probe PCR
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概要
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Quenching probe (QProbe) PCR-based methods for quantifying the abundance of Rhodocyclus-related and actinobacterial polyphosphate-accumulating organisms (PAOs) in an enhanced biological phosphorus removal (EBPR) process are described. PCR primer-QProbe sets specific for the 16S rRNA genes of Rhodocyclus-related PAOs (RPAOs) and actinobacterial PAOs (APAOs) were newly designed, and their specificity tested using database searches, a selection of pure cultures, and sludge DNA. The sets were found to be specific for most of the target sequences. QProbe PCR with the newly designed sets was used to enumerate the 16S rRNA genes of RPAOs and APAOs in a laboratory-scale EBPR sludge. The copy number of 16S rRNA genes of RPAOs was larger than that of APAOs before the phosphorus removal performance of the reactor deteriorated, and the amount of both PAOs decreased in the deterioration period. The approaches developed in this study may become a powerful tool for a high-throughput analysis of PAO abundance in EBPR processes.
著者
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Uchiyama Hiroo
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Noda Naohiro
National Institute of Advanced Industrial Science and Technology
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Uchiyama Hiroo
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Tanaka Hideo
Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive
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OKUNUKI SUGURU
Graduate School of Life and Environmental Sciences, University of Tsukuba
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NAKAMURA KAZUNORI
National Institute of Advanced Industrial Science and Technology (AIST)
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KAWAHARASAKI MAMORU
National Institute of Advanced Industrial Science and Technology (AIST)
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TANAKA HIDEO
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Noda Naohiro
National Institute Of Advanced Industrial Science And Technology (aist)
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Tamaki Hideyuki
Inst. For Biological Resources And Functions National Inst. Of Advanced Industrial Sci. And Technol.
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Okunuki Suguru
Graduate School Of Life And Environmental Sciences University Of Tsukuba:national Institute Of Advan
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Tamaki Hideyuki
Aist
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Kawaharasaki Mamoru
National Institue Of Bioscience And Human-technology Agency Of Industrial Science And Technology
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Uchiyama Hiroo
Life Sciences And Bioengineering Graduate School Of Life And Environmental Sciences University Of Ts
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Tamaki Hideyuki
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Tamaki Hideyuki
Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a
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Tanaka Hideo
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Tamaki Hideyuki
Institute Of Applied Biochemistry University Of Tsukuba
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Uchiyama Hiroo
Life Sciences And Bioengineering Graduate School Of Life And Environmental Sciences University Of Ts
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Tanaka Hideo
Graduate School of Economics, Kyoto University
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