Measurement of Viable Plant Cell and Protoplast Concentrations with Specialized Fluorometer
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概要
- 論文の詳細を見る
A specialized fluorometer was developed for measurement of fluorescence intensities of plant cell and protoplast suspensions after staining with fluorescein diacetate. With this apparatus, the suspensions in a cuvette can be mixed almost homogeneously at an optimal agitation speed (800 rpm). At this agitation speed, stable fluorescence intensities were obtained because there was neither cell and protoplast sedimentation nor destruction. For both Nicotiana tabacum BY2 and Catharanthus roseus L., Iinear relationships between the fluorescence intensities of cell/protoplast suspensions and viable cell/protoplast concentrations (from microscopic counting) were obtained. Viabilities of protoplasts calculated using fluorometry were proportional to their colony-forming efficiencies. With this apparatus, viable plant cell and protoplast concentrations can be measured with ease and accuracy.
- 社団法人日本生物工学会の論文
- 1994-05-25
著者
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Aoyagi Hideki
Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive
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Aoyagi Hideki
Institute Of Applied Biochemistry University Of Tsukuba
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Tanaka Hideo
Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive
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Tanaka Hideo
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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