Efficient Production of Chitinase by Wasabia japonica Protoplasts Immobilized in Double-Layered Gel Fibers

スポンサーリンク

概要

• 論文の詳細を見る

• Double-layered gel fibers consisting of an inner layer in which Wasabia japonica protoplasts were immobilized and an outer layer of calcium alginate was prepared and used for chitinase production in Erlenmeyer flasks. Immobilized protoplasts could be cultivated in shake cultures at low osmotic pressure without disruption. The chitinase productivity by the protoplasts immobilized in double-layered gel fiber (2.0 U/ml at 5 d) was much higher than that of the immobilized cells (0.36 U/ml at 5 d). On the third day of cultivation, cell wall regeneration in the immobilized protoplasts was detected under light microscope. This implies that prevention of cell wall regeneratino is a pre-requisite for long term process with protoplasts. When 2,6-dichlorobenzonitile was added to the broth, cellulose cell wall synthesis was inhibited, and active protoplasts were maintained for a long time without cell wall regeneration. The chitinase productivity increased significantly when the alginate-immobilized protoplasts were cultivated under pure oxygen aeration. Similar results were also obtained in a bubble column reactor. At high chitinase concentrations, product inhibition was observed. The chitinase production in the batch culture of the immobilized protoplasts could be repressented by the following equation, dP/dt=η_P・(1-P/P_m^<0.29>, where P=chitinase concentration (U-enzyme/l-broth), P_m=limit chitinase concentration (U-enzyme/l-broth) and η_P=maximum chitinase production rate (U-enzyme/l-broth・d). During chitinase production by the immobilized protoplasts, product inhibition was observed from the start of the culture and in order to maintain the chitinase productivity above 80% of the maximum productivity, it was necessary to keep the chitinase concentration in the broth lower than 1.8 U/ml. A system for continuous production with simultaneous recovery of chitinase was therefore developed. A production column containing W. japonica protoplasts immobilized in double-layered gel fibers was coupled to a chitin columm and by circulating the fermentation broth between the two columns, continuous production with simultaneous recovery of chitinase was successfuly carried out. With this system, it was possible to maintain high and stable chitinase production for 25 d and a high amount of chitinases (26,000 U) was obtained.

• 社団法人日本生物工学会の論文
• 1996-05-25

著者

• Aoyagi H

  Institute Of Applied Biochemistry University Of Tsukuba

• Aoyagi Hideki

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• Aoyagi Hideki

  Institute Of Applied Biochemistry University Of Tsukuba

• Aoyagi H

  Univ. Tsukuba Ibaraki Jpn

• Tanaka Hideo

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• Yamashita Takanori

  Institute Of Applied Biochemistry University Of Tsukuba

• YAMAMOTO YOSHINARI

  Gakken Co. Ltd.,

• FUKUNAGA YUKIO

  Gakken Co. Ltd.,

• Tanaka H

  Institute Of Applied Biochemistry University Of Tsukuba

• Fukunaga Yukio

  Gakken Co. Ltd.

• Yamamoto Yoshinari

  Gakken Co. Ltd.

• Tanaka Hideo

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

関連論文

• Quantification of Rhodocyclus-Related and Actinobacterial Polyphosphate-Accumulating Organisms in an Enhanced Biological Phosphorus Removal Process Using Quenching Probe PCR
• 2D11-4 Production of (R)-3-hydroxybutyric acid by Cupriavidus necator
• 3H10-4 Cyclic production of poly(3-hydroxybutyrate) and D-3-hydroxybutyrate by A. latus and R. eutropha
• 3E13-5 Production of cell biomass and bioactive compounds by microalgal cultivation systems
• 1149 A simple tubular photobioreactor with internal static mixers for improved mass transfer characteristics
• Development of an Apparatus for Monitoring Protoplast Isolation from Plant Tissues Based on Both Dielectric and Optical Methods
• Phylogenetic Relationship of Symbiotic Archaea in the Gut of the Higher Termite Nasutitermes takasagoensis Fed with Various Carbon Sources
• Estimation of Cell Biomass in Plant Cell Suspensions by the Osmotic Pressure Measurement of Culture Broth
• Bacterial Alginate Lyase Gene : Nucleotide Sequence and Molecular Route for Generation of Alginate Lyase Species
• Promotion of Germination and Shoot Elongation of Some Plants by Alginate Oligomers Prepared with Bacterial Alginate Lyase
• Application of Maxblend Fementor^ for Microbial Processes
• Scale-up of Bialaphos Production
• Effect of Oxygen Partial Pressure on Bialaphos Synthesis, Sugar Metabolism and the Activity of Tricarboxylic Acid Cycle Enzymes in Submerged Culture of Streptomyces hygroscopicus : Studies on Production of Bialaphos (III).
• Accumulation of Bialaphos Intermediates under High Oxygen Partial Pressure and Their Conversion to Bialaphos : Studies on the Production of Bialaphos(IV).
• Relationship between Sugar Consumption and Tricaboxylic Acid Cycle Enzyme Activity in a High Bialaphos-Producing Strain : Studies on the Production of Bialaphos(II).
• Efficient Production of Ethanol by Cells Immobilized in Loofa (Luffa cylindrica) Sponge
• Loofa (Luffa cylindrica) Sponge as a Carrier for Microbial Cell Immobilization
• Fermentation of Bacillus subtilis ATCC 6633 and Production of Subtilin in Polyethylene Glycol/Phosphate Aqueous Two-Phase Systems
• Control of Autolysis of Bacillus subtilis for Selective Production of the Intracellular Enzyme Glucose-6-Phosphate Dehydrogenase in Aqueous Two-Phase Systems
• EVALUATION OF SURFACE HYDROPHOBICITIES DURING REFOLDING PROCESS OF CARBONIC ANHYDRASE USING AQUEOUS TWO-PHASE PARTITIONING SYSTEM
• Purification and Characteristics of Chitinase Secreted by Cultured Wasabia japonica Cells
• Measurement of Fresh and Dry Densities of Suspended Plant Cells and Estimation of Their Water Content
• Acetylation of Loofa(Luffa cylindricd) Sponge as Immobilization Carrier for Bioprocesses Involving Cellulase(BIOCHEMICAL ENGINEERING)
• Development of a Novel System for Producing Ajmalicine and Serpentine Using Direct Culture of Leaves in Catharanthus roseus Intact Plant
• Efficient Production of Chitinase by Immobilized Wasabia japonica Cells in Double-Layered Gel Fibers
• A New Scale-up Method Based on the Effect of Ventilation on Aerated Fermentation Systems : The Effect of Ventilation on Aerated Fermentation System (I).
• Mechanism of Fermentation Performance of Zymomonas mobilis under Oxygen Supply in Batch Culture : Fermentation Performance of Zymomonas mobilis against Oxygen Supply (II).
• Fermentative Ability of Zymomonas mobilis under Various Oxygen Supply Conditions in Batch Culture : Fermentation Performance of Zymomonas mobilis against Oxygen Supply (I).
• Invertase Production by Saccharomyces cerevisiae Protoplasts Immobilized in Strontium Alginate Gel Beads
• Development of a Novel Box-Shaped Shake Flask with Efficient Gas Exchange Capacity
• Analysis of Gene Expression in Yeast Protoplasts Using DNA Microarrays and Their Application for Efficient Production of Invertase and α-Glucosidase (BIOCHEMICAL ENGINEERING)
• Efficient Production of Chitinase by Wasabia japonica Protoplasts Immobilized in Double-Layered Gel Fibers
• Development of an Optical Method for Monitoring Protoplast Formation from Cultured Plant Cells
• Turbidimetric Measurement of Cell Biomass of Plant Cell Suspensions
• Indole Alkaloids Production by Catharanthus roseus Protoplasts with Artificial Cell Walls Containing of Guluronic Acid Rich Alginate Gel
• A Novel Internally Illuminated Stirred Tank Photobioreactor for Large-Scale Cultivation of Photosynthetic Cells
• Light Supply Coefficient : A New Engineering Parameter for Photobioreactor Design
• Kinetic Study on Light-Limited Batch Cultivation of Photosynthetic Cells
• Effect of Cell Movement by Random Mixing between the Surface and Bottom of Photobioreactors on Algal Productivity
• Measurement of Viable Plant Cell and Protoplast Concentrations with Specialized Fluorometer
• Production of 5'-Phosphodiesterase by Catharanthus roseus Cells Promoted by Heat-Degraded Products Generated from Uronic Acid
• Utilization of Spent Sawdust Matrix after Cultivation of Grifola frondosa as Substrate for Ethanol Production by Simultaneous Saccharification and Fermentation
• 3D09-4 Bioconversion of raw cassava starch in a novel alternating liquid phase-air phase bioreactor with cells immobilized in loofa (Luffa cylindrica) sponge
• Use of Food Additives to Prevent Contamination during Fermentation Using a Co-Immobilized Mixed Culture System
• Synergistic Effect of Active Oxygen Species and Alginate on Chitinase Production by Wasabia japonica Cells and Its Application
• The Ecological Role of a Proteolytic Psychrophile, Flavobacterium limicola Belonging to the Cytophaga-Flavobacteria Cluster, in the Decomposition of Particulate Organic Nitrogen in Freshwater Sediment
• 霞ヶ浦における密度流拡散装置を用いた大規模汚濁湖沼底質改善
• Characteristics of Loofa(Luffa cylindrica)Sponge as a Carrier for Plant Cell Immobilization
• Breeding of Bialaphos Producing Strains from a Biochemical Engineering Viewpoint : Studies on the Production of Bialaphos (I).
• A Simple Method of Fuzzy Modeling for a Microorganism Reaction
• Effect of Ventilation on the Productin of Acetaldehyde by Zymomonas mobilis : The Effect of Ventilation on Aerated Fermentation System (II).
• A Novel Immobilization Method for Prevention of Cell Leakage from the Gel Matrix
• Co-Immobilization of Three Strains of Microorganisms and Its Application in Ethanol Production from Raw Starch under Unsterile Conditions
• Characteristics of Escherichia coli HB101 and Pseudomonas putida PpY101 Harboring a Recombinant Plasmid with Tandem Insertion of the Mercury Resistance Operon
• High Density Cultivation of Plant Cells in a New Aeration-Agitation Type Fermentor, Maxblend Fermentor^[○!R]
• Correlation between necl Gene Copy Number Detected in Soils by Quantitative Competitive Quenching Probe PCR and Incidence of Potato Common Scab Disease
• The Flexure Torsion Flutter of Aerofoils in Cascade : 2nd Rcport, Relations between Flutter Velocity and Cascade Conditions
• A Simplified Method to Measure Unsteady Forces Acting on the Vibrating Blades in Cascade
• Stall Flutter of a Thin Aerofoil with Leading Edge Separation
• The Flexure-Torsion Flutter of Aerofoils in Cascade : 1st Report, Computation and Experiment of Unsteady Aerodynamic Derivatives
• Control of Diacetyl Precursor [α-Acetolactate] Formation during Alcohol Fermentation with Yeast Cells Immobilized in Alginate Fibers with Double Gel Layers
• PB-61 Methanogen diversity and methanogenic potential in natural gas fields(Community structure,Poster presentation B)
• Influence of Feed Components on Symbiotic Bacterial Community Structure in the Gut of the Wood-Feeding Higher Termite Nasutitermes takasagoensis
• A novel laccase with urate oxidation activity from Lysobacter sp. T-15
• Isolation and Identification of Bacterial Strains Producing Diffusible Growth Factor(s) for Catellibacterium nectariphilum strain AST4^T
• Culture-Dependent and Independent Analyses of the Microbial Communities Inhabiting the Giant Duckeweed (Spirodela polyrrhiza) Rhizoplane and Isolation of a Variety of Rarely Cultivated Organisms within the Phylum Verrucomicrobia
• Development of a Genotyping Method for Potato Scab Pathogens Based on Multiplex PCR
• Coprinopsis cinerea from rice husks forming sclerotia in agar culture
• Study of Flow Around a Rotating Circular Cylinder
• Necrosis Gene-Based Monitoring and Control of Potato Scab Disease
• IGTC-S1 A SURVEY ON GAS TURBINE TECHNOLOGY AND RESEARCH WORK IN JAPAN
• Night Biomass Loss and Changes in Biochemical Composition of Cells during Light/Dark Cyclic Culture of Chlorella pyrenoidosa
• Flow Uniformalizing Effect of Damping Screen in Pipe Flow : The Case of S-Shape Velocity Distribution
• Effect of Interference between Moving Blade Rows on Cascade Flutter : 1st. report : Experiment on Compressor Cascade in Flexure Mode
• A Six-well Plate Method : Less Laborious and Effective Method for Cultivation of Obligate Anaerobic Microorganisms
• Microbial Community Analysis in the Roots of Aquatic Plants and Isolation of Novel Microbes Including an Organism of the Candidate Phylum OP10
• Primary simple assays of cellulose-degrading fungi

もっと見る 閉じる

スポンサーリンク