Accumulation of Bialaphos Intermediates under High Oxygen Partial Pressure and Their Conversion to Bialaphos : Studies on the Production of Bialaphos(IV).

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In bialaphos(a herbicide)fermentation by Streptomyces hygroscopicus, production of bialaphos is greatly reduced when the partial pressure of oxygen is increased. In order to determine the inhibitory effect of high oxygen partial pressure, the culture filtrate was analyzed revealing that large amounts of two possible biosynthetic intermediates of bialaphos were accumulated. These two compounds were identified to be C-P compounds having an amino group, one as a demethyl derivative of bialaphos(MP-102)and the other a demethyl derivative of AMPB[dealaninated bialaphos](MP-101). It was also demonstrated that these demetyl derivatives were converted to bialaphos when they were added to the culture under low oxygen partial pressure, whereas the conversion was suppressed under high oxygen partial pressure. As a conclusion, methylation at the final step of the biosynthetic process of bialaphos did not proceed under high oxygen partial pressure, resulting in a low bialaphos concentration due to the accumulation of the demethyl derivatives.
社団法人日本生物工学会の論文
1991-06-25