Development of a Genotyping Method for Potato Scab Pathogens Based on Multiplex PCR
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概要
- 論文の詳細を見る
Scab disease significantly damages potato and other root crops. Streptomyces scabiei, S. acidiscabiei, and S. turgidiscabiei are the best-known causal agents of this disease. We have developed a novel genotyping method for these potato scab pathogens using multiplex PCR, whose benefits include rapid and easy detection of multiple species. We designed a species-specific primer set (6 primers, 3 pairs) for the 16S rRNA genes and 16S–23S ITS regions of these potato scab pathogens. The specificity of the primer set was confirmed by testing 18 strains containing potato scab pathogens, other Streptomyces species, and strains of other genera. The application of the developed method to potato field soil and potato tissue samples resulted in the clear detection and identification of pathogens. Since this method is applicable to a large number of environmental samples, it is expected to be useful for a high-throughput analysis of soil and plant tissues of scab disease.
- 社団法人 日本農芸化学会の論文
- 2008-09-23
著者
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Tanaka Hideo
Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive
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Kamagata Yoichi
AIST
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Koyama Osamu
Research And Development Laboratory Kankyo Engineering Co. Ltd
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Tamaki Hideyuki
Aist
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Kamagata Yoichi
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Kamagata Yoichi
Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a
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Tamaki Hideyuki
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Tamaki Hideyuki
Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a
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Koyama Osamu
Research And Development Laboratory Nippon Steel Kankyo Engineering Corporation
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Tagawa Masahiro
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Manome Akira
Research And Development Laboratory Nippon Steel Kankyo Engineering Corporation
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Tamaki Hideyuki
Institute For Biological Resources & Functions National Institute Of Advanced Industrial Science
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Kamagata Yoichi
Institute For Biological Resources & Functions National Institute Of Advanced Industrial Science
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Tamaki Hideyuki
Institute Of Applied Biochemistry University Of Tsukuba
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