Effect of Ventilation on the Productin of Acetaldehyde by Zymomonas mobilis : The Effect of Ventilation on Aerated Fermentation System (II).

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概要

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• The production of acetaldehyde, a flavoring agent in food, by Zymomonas mobilis was carried out in batch culture. The volatilization rate constant (k_v) of acetaldehyde and the initial volumetric oxygen transfer coefficient (k_Lα^0)in an Erlenmeyer flask with a cotton-plug (cotton-flask) and an aerated-flask with a forced-air system (aerated-flask) were measured. The culture environment in the aerated-flask was found to be very different from that in the cotton-flask. Cell growth in a cotton-flask was strongly inhibited, making practical acetaldehyde production in cotton-flask very difficult. On the other hand, acetaldehyde production in the aerated-flask increased while the fermentation time decreased with increases in the air flow rate. The k_v value of acetaldehyde in a jar fermentor was affected mainly by air flow rate. By considering both the oxygen transfer rate and the ventilation effect on the culture, it was possible to scale-up form the aerated-flask to a jar fermentor. In the jar fermentor, production of acetaldehyde and growth inhibiton by acetaldehyde were affected mainly by the k_Lα^0 and k_v values, respectively. The overall production of acetaldehyde in the jar fermentor under the optimum k_Lα^0 and k_v conditions was 6.64 g/l (Y_<P/S> : 0.27), which was about 1.5 times higher than the maximum concentration obtained in the aerated-flask.

• 社団法人日本生物工学会の論文
• 1992-04-25

著者

• Tanaka Hideo

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• ISHIKAWA HIDENORI

  Institute of Applied Biochemistry, University of Tsukuba

• Ishikawa Hidenori

  Institute Of Applied Biochemistry University Of Tsukuba

• Tanaka Hideo

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

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