High Density Cultivation of Plant Cells in a New Aeration-Agitation Type Fermentor, Maxblend Fermentor^[○!R]

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The applicability of a new aeration-agitation type fermentor with a grid-paddle type impeller and a spiral-sparger, Maxblend Fermentor ^[○!R] (MBF) for high density cultivaiton of plant cells, was investigated. The MBF showed a high capacity for oxygen supply and extremely low hydrodynamic stress in aeration and mixing compared with a conventional fermentor (CF). When Oryza sativa cells were cultivated at a k_Lα of 20h^<-1>, a high cell density cultivation of about 30g dry cell weight per liter was accomplished in both fermentors and there were few differences in culture performance between the two. On the contrary, considerable differences were observed when Catharanthus roseus cells, which seemed to be sensitive to physical stress, were cultivated at a k_Lα of 20h^<-1> in both fermentors. The MBF exhibited excellent cell growth characteristics, achiving about 19g dry cell weight per liter, because of its superior oxygen supply and low hydrodynamic stress in aeration and mixing in highly viscous cultures containing high density cells. In CF only about 9.5g dry cell weight per liter was achieved because of its high hydrodynamic stress.
社団法人日本生物工学会の論文
1993-01-25