Optimization and Scale-Up of γ-Linolenic Acid Production by Mortierella ramanniana MM 15-1,a High γ-Linolenic Acid Producing Mutant

概要

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Optimum conditions for cultivation of Mortierella ramanniana mutant MM 15-1 were investigated with an aim of producing a lipid which contains high γ-linolenic acid (GLA). The optimum initial glucose concentration and pH control level were 300 g/l and 4.0,respectively. Although both pellet and filamentous forms of this filamentous forms. The effects of culture conditions on pellet formation were therefore investigated in a 30-l jar fermentor. The results showed that with an inoculum spore concentration of 5.0×10^3/ml and an agitation speed of 800 rpm, 0.15-0.5 mm pellets were formed and consequently, the GLA content of the lipid increased to a very high value of 18.3%. Furthermore, when this process was scaled up to a 10-kl fermentor, using the impeller tip velocity as the scale-up parameter, both the pellet formation and the lipid production as well as the GLA content of the lipid were consistent with those obtained in the 30-l jar fermentor.
社団法人日本生物工学会の論文
1996-10-25